Gw843682x

Nocodazole (M1404; Sigma Aldrich) was used at 3.3 μM, MG132 at 10 μm (C2211, Sigma Aldrich), GW843682X at 1 μm (2977, Tocris, Bristol, UK), BI 6727 at 100 nM (S2235, Stratech Scientific, Suffolk, UK), Hesperadin at 100 nM or 500 nM (2096, Axon Medchem, Groningen, The Netherlands), Reversine at 100 nM or 500 nM (10004412, Cayman Chemical, Michigan, USA), 5-Iodotubercidin at 10 μm (HY-15424, Axon Medchem). DMSO was used as a control in the experiments.

The fifty-five protein kinase inhibitors (PKIs) were purchased from following sources: BML-275, FR 180204, IKK16, GW 843682X, NSC 109555, NU7441, PD407824, PF 573228, SB 218078, TCS PIM-1-1, TCS PIM-1-4a, and TPCA-1 from Tocris Biosciences (Bristol, UK); indirubin-3′-monoxime and Ro-31-8220 from Calbiochem (San Diego, CA, USA); A-769662, bosutinib, chelerythrine, CP690550, fasudil, gefitinib, imatinib, nilotinib, PKC412, roscovitine, SNS-314, and tozasertib from LC Laboratories (Woburn, MA, USA); AT7867, AT9283, AZD1152, AZD1480, BI 2536, BIX 02189, CHIR-99021, CI-1040, CYC116, danusertib, enzastaurin, GDC-0879, INCB018424, JNJ-7706621, KU-55933, LY2228820, MLN8237, PD-0325901, PF-4708671, PLX-4032, PLX-4720, SB216763, SNS-032, SP600125, VX-702, Y-27632, and ZM447439 from Selleck Chemicals (Houston, TX, USA); U0126 from Promega (Madison, WI, USA); TBCA from Millipore (Burlington, MA, USA).
All TNBC cells in this study were obtained from the American Type Culture Collection (Manassas, VA, USA). The cultured cells were monitor by trypan blue cell counting as described previously [58 (link)].

Primary and secondary antibodies used for immunodetection are summarized in Supplementary Table S2. Gö6976, SP600125 and NSC-87877 were purchased from Calbiochem (Merck Millipore), CKI-7 from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA) and GW843682X from Tocris Bioscience (Tocris Bioscience, Bristol, United Kingdom).