For intracellular staining of arginase-1, cells were fixed and permeabilized with Fix/perm buffer (eBioscience), washed and then stained with anti-arginase-1 (BD biosciences), as described before (28 (link)).
Anti arginase 1
Manufactured by BD
Sourced in United States
Anti-arginase-1 is a laboratory reagent used to detect and quantify the presence of arginase-1, an enzyme involved in the urea cycle. It can be used in various research and diagnostic applications, but its specific intended use should be determined by the user.
Automatically generated - may contain errors
Lab products found in correlation
Fix perm buffer, by Thermo Fisher Scientific (1 mentions)
Anti fibronectin, by Abcam (1 mentions)
Anti α sma, by Agilent Technologies (1 mentions)
Avidin biotin block, by Vector Laboratories (1 mentions)
Anti pd 1, by Thermo Fisher Scientific (1 mentions)
Molecular probe, by Thermo Fisher Scientific (1 mentions)
Anti tcrβ, by BD (1 mentions)
Anti cd8, by BD (1 mentions)
Anti ly6g, by BD (1 mentions)
Anti mouse f4 80, by BD (1 mentions)
Anti gr1, by BioLegend (1 mentions)
Anti ly6c, by BD (1 mentions)
Cd11b, by BioLegend (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti phospho atf2, by Cell Signaling Technology (1 mentions)
Anti c jun, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
5 protocols using anti arginase 1
1
Intracellular Arginase-1 Staining
2
Immunohistochemical Analysis of Lung Tissue
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Immunohistochemistry was performed at McMaster’s Histology Core Facility as previously described15 (link). Briefly, formalin-fixed tissues were cut at 5 µm sections and stained with Masson’s trichrome for ECM, anti-αSMA (Dako, cat# M0851) for the identification of αSMA-positive myofibroblasts and anti-fibronectin (Abcam, cat# ab2413) for the assessment of pro-fibrotic factors. Lung tissue sections were also stained with anti-arginase-1 (BD Biosciences, cat# 610708) for the identification of alternatively activated macrophages. We used the Dako ARKTM (Animal Research Kit) Peroxidase (cat# K3954) to execute immunohistochemical stains involving mouse primary antibodies on mouse lung tissues. As for tissues that contain endogenous biotin (such as liver and kidney and other tissues), a step involving avidin/biotin block (Vector Laboratories, cat# SP2001) was conducted prior to immunohistochemical staining of the lung tissues. Diluent and IgG negative controls as well as non-treated tissue controls were included to ensure precision of the staining protocol and optimal antibody concentrations.
3
Detailed Phenotypic Analysis of Immune Cells
4
Western Blot Analysis of Signaling Proteins
5
Quantifying Neurobiological Markers in Mouse Brain
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The mouse brain was extracted using protein extraction buffer (T-PER, Thermo Fisher, USA) containing protease and phosphatase inhibitors (Thermo Fisher, USA). Equal amounts of protein extracts (20µg) were separated by SDS-PAGE and transferred onto a nitrocellulose lter (NC) membrane (GE Healthcare Life Sciences, USA). After being blocked with 5% non-fat milk, membranes were incubated at 4°C overnight with primary antibodies (all at 1:1000) as follows: anti-β-actin (Abcam, USA), anti-p-mTOR(Abcam, USA), anti-GDNF(Abcam, USA), anti-BNDF(Abcam, USA), anti-CNTF(Abcam, USA), anti-Bcl-2(Abcam, USA), anti-Bax (Abcam, USA), anti-p110α-PI3K (C73F8) (Cell Signaling Technology, MA), anti-p-Akt (Ser473) (Cell Signaling Technology, MA), anti-β-catenin (Cell Signaling Technology, MA), anti-Fzd1 (R&D system, USA), anti-NLRP3 (R&D system, USA), anti-WNT1 (ABGENT, USA), anti-iNOS/NOS(BD, USA) or anti-Arginase1 (BD, USA). After being washed in TBST, the immunoblots underwent incubation with horseradish peroxidase-conjugated secondary antibodies (Jackson Lab, USA) for 1h. Bands were visualized and analyzed using a ChemiDoc XRS (Bio-Rad, USA) and Image Lab(Bio-Rad, USA).
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