Anti cd41

Manufactured by Abcam

Sourced in United States

Anti-CD41 is a protein that binds to the CD41 antigen. CD41 is a cell surface glycoprotein that plays a role in platelet activation and aggregation. This product can be used for research purposes to study the function and expression of CD41.

Automatically generated - may contain errors

Lab products found in correlation

3 3 diaminobenzidine tetrahydrochloride dab, by Vector Laboratories (1 mentions) Anti collagen type 2, by Merck Group (1 mentions) Anti type 1 collagen antibody, by Southern Biotech (1 mentions) Axio imager m2, by Zeiss (1 mentions) Alexa fluor 594 conjugated anti mouse igg antibody, by Abcam (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated anti rabbit igg antibody, by Abcam (1 mentions) Alexa fluor 594 conjugated anti rabbit igg antibody, by Abcam (1 mentions) Alexa fluor 488 conjugated anti mouse igg antibody, by Abcam (1 mentions) Anti nlrp3, by Abcam (1 mentions) Pluronic f68, by Merck Group (1 mentions) Anti epcam, by R&D Systems (1 mentions) Kx 21n, by Sysmex (1 mentions) Anti phospho cofilin, by Cell Signaling Technology (1 mentions) Trizol reagent, by Selleck Chemicals (1 mentions) Anti phospho mek1 2, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti cofilin, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti cd42b, by Abcam (1 mentions)

Close spelling variants of this product

Anti-CD41 antibody (ab134131) Anti-mouse CD41

5 protocols using anti cd41

Chondrocyte Phenotypic Analysis via IHC and IF

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chondrocytes cultured in D, M, DF, or MF (CCs-D, CCs-M, CCs-DF, or CCs-MF) were plated on cover slips, cultured for 2 d, fixed with 3.7% formalin in PBS, and permeabilized with 0.2% Triton X-100. For immunohistochemical (IHC) staining, anti-type I collagen antibody (Southern Biotech Associates) was applied to the cover slips at 4 °C overnight, and the results were developed with 0.1% 3,3′-diaminobenzidine tetrahydrochloride (DAB; Vector Laboratories, Burlingame, CA, USA) in PBS for 5 min. Nuclei were counterstained with hematoxylin. For immunofluorescence staining, cover slips were blocked with 20% normal goat serum and then reacted with anti-type II collagen (Millipore; cat. # MAB8887, monoclonal, clone # 6B3), anti-α-SMA (smooth muscle actin) (DAKO, Glostrup, Denmark; cat. # M0851, monoclonal, clone # 1A4), anti-CD41 (Abcam, Cambridge, MA, USA; cat. # ab63983, polyclonal), or anti-CD29 (DAKO; cat. #M0889, monoclonal, clone # K20). Fluorescein isothiocyanate–labeled goat anti-mouse IgG (Vector Laboratories) was used as a secondary antibody, and nuclei were counterstained for 5 min with 4′,6-diamidino-2-phenylindole.

Lee J., Lee J.Y., Chae B.C., Jang J., Lee E, & Son Y. (2017). Fully Dedifferentiated Chondrocytes Expanded in Specific Mesenchymal Stem Cell Growth Medium with FGF2 Obtains Mesenchymal Stem Cell Phenotype In Vitro but Retains Chondrocyte Phenotype In Vivo. Cell Transplantation, 26(10), 1673-1687.

+ Open protocol

+ Expand

Murine Pulmonary Vascular Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Staining of lung sections was performed to analyze the phenotypic differences between the control and experimental groups. Specifically, hematoxylin and eosin (H/E) staining was used to evaluate differences in murine pulmonary vasculature. Immunohistochemical staining for thrombocyte-specific antigen (anti-CD41, Abcam, Cambridge, UK) was used to determine the presence of platelet aggregates. Martius Scarlet Blue (MSB) staining was used to determine the presence of mature fibrin strands.
The concentration of CFSE-labelled microparticles within lung sections was quantified under immunofluorescent microscopy. Eight random captures of each slide were taken at the lowest magnification using imaging software ZEN version 1.1.2.0 on Axio Imager M2 microscope (Carl Zeiss AG, Jena, Germany). Light microscopy was similarly used to analyze murine lungs stained with H/E, thrombocyte-specific antigen, and MSB.

Kim Y., Goodman M.D., Jung A.D., Abplanalp W.A., Schuster R.M., Caldwell C.C., Lentsch A.B, & Pritts T.A. (2019). Microparticles from Aged Packed Red Blood Cell Units Stimulate Pulmonary Microthrombus Formation via P-Selectin. Thrombosis research, 185, 160-166.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Inflammasome Components

Check if the same lab product or an alternative is used in the 5 most similar protocols

Platelets or PMNs were fixed and incubated with antibodies including anti-NLRP3 (Abcam, 1:100, catalog no. ab4207), anti-ASC (Novusbio, 1:100, catalog no. NBP1-78978), anti-CD41 (Abcam, 1:200, catalog no. ab134131), Alexa Fluor 488 anti-mouse CD41 (BioLegend, 1:200, catalog no. 133908), Hoechst (Thermo Fisher Scientific, 1:3,000, catalog no. H3570), anti-myeloperoxidase (Genetx, 1:200, catalog no. gtx75318), anti-myeloperoxidase (Abcam, 1:200, catalog no. ab208670) and anti-histone H3 (citrulline R2 + R8 + R17, 1:200, Abcam, catalog no. ab5103) at 4 °C overnight. Secondary antibodies included Alexa Fluor 647-conjugated anti-goat antibody (Abcam, 1:200, catalog no. ab150135), Alexa Fluor 647-conjugated anti-rabbit IgG antibody (Abcam, 1:200, catalog no. ab150079), Alexa Fluor 594-conjugated anti-rabbit IgG antibody (Abcam, 1:200, catalog no. ab150084), Alexa Fluor 594-conjugated anti-mouse IgG antibody (Abcam, 1:200, catalog no. ab150116), Alexa Fluor 488-conjugated anti-goat IgG antibody (BioLegend, 1:200, catalog no. 405508), Alexa Fluor 488-conjugated anti-rabbit IgG antibody (Abcam, 1:200, catalog no. ab150077) and Alexa Fluor 488-conjugated anti-mouse IgG antibody (Abcam, 1:200, catalog no. ab150113). Cells were captured using immunofluorescence confocal microscopy (×63 oil immersion lens, Leica SP8).

Su M., Chen C., Li S., Li M., Zeng Z., Zhang Y., Xia L., Li X., Zheng D., Lin Q., Fan X., Wen Y., Liu Y., Chen F., Luo W., Bu Y., Qin J., Guo M., Qiu M., Sun L., Liu R., Wang P., Hwa J, & Tang W.H. (2022). Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis. Nature Cardiovascular Research, 1(8), 732-747.

+ Open protocol

+ Expand

Microfluidic Isolation of Circulating Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The first-stage hydrodynamic sorting (DLD) devices were designed at Massachusetts General Hospital and fabricated by Silex with deep reactive ion etching on silicon wafers as described previously^{20 ,37 (link)}. The DLD array consists of 24 parallel channels that are 150 μm in depth. The gap size between posts and the vertical pitch are both 32 μm, the horizontal pitch is 56 μm, and the row shift fraction is 1/60. The device was sealed with anodically bonded glass cover to form the microfluidic chamber. A custom polycarbonate manifold was used to form the fluidic connections to the microchip. The second-stage HB-Chips were made of PDMS bonded to glass substrates using soft lithography techniques, and functionalized with anti-EpCAM (20 μg/mL, R&D Systems) or anti-CD41 (20 μg/mL, Abcam) using avidin-biotin chemistry as described^{19 (link)}.
Whole blood was first processed through the DLD chip with co-flow of buffer (1% w/v Pluronic F-68, Sigma; in PBS). The product contains highly enriched nucleated cells in buffer with complete blood counts (Sysmex KX-21N) that indicate >2 log-depletion of platelets. This product was then processed using the CD41 or EpCAM HB-Chip at 1.14 ± 0.24 mL/hr for CTC isolation.

Jiang X., Wong K.H., Khankhel A.H., Zeinali M., Reategui E., Phillips M.J., Luo X., Aceto N., Fachin F., Hoang A.N., Kim W., Jensen A.E., Sequist L.V., Maheswaran S., Haber D.A., Stott S.L, & Toner M. (2017). Microfluidic isolation of platelet-covered circulating tumor cells. Lab on a chip, 17(20), 3498-3503.

+ Open protocol

+ Expand

Platelet Activation and Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

TPO (Cat. no. 1624) was purchased from Tocris (Minneapolis, MN, USA). JYBL-1 was from Sigma-Aldrich (Solarbio Life Sciences), and TRIzol^® reagent was purchased from Selleckchem (Invitrogen). SYBR™ Premix Ex Taq II was purchased from Thermo Fisher Scientific (Waltham USA, MA). CREG1 was purchased from Sigma-Aldrich (09015-1B7) and HUABIO (ER61836). The DyLight™ 488 conjugation kit, and anti-CD42a, anti-CD42b, and anti-CD41 antibodies, were purchased from Abcam (Cambridge, USA). Anti-GAPDH, anti-cleaved caspase-3, anti-phospho-MEK1/2, anti-MEK1/2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-ERK1/2, anti-p38, anti-phospho-p38 (Thr180/Tyr182), anti-phospho-PAK1/2 (Ser209), anti-phospho-LIMK1, anti-phospho-cofilin, anti-total PAK, anti-cofilin, and anti-total LIMK1 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). PE-CD41 was obtained from BD Biosciences, and FITC-CD42b was obtained from Emfret Analytics. U0126 and propidium iodide were obtained from Sangon Biotech (Shanghai, China).

Song H., Li J., Peng C., Liu D., Mei Z., Yang Z., Tian X., Zhang X., Jing Q., Yan C, & Han Y. (2023). The role of CREG1 in megakaryocyte maturation and thrombocytopoiesis. International Journal of Biological Sciences, 19(11), 3614-3627.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!