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Cd25 7d4

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The CD25 (7D4) is a monoclonal antibody that binds to the CD25 antigen, also known as the IL-2 receptor alpha chain. It is commonly used in flow cytometry applications to identify and quantify CD25-expressing cells.

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Lab products found in correlation

Facsdiva software, by BD (2 mentions) Fjk 16s, by BD (2 mentions) Mac 1 m1 70, by BD (1 mentions) Cd4 gk1, by BD (1 mentions) Cd8 53 6, by BD (1 mentions) Cd44 im7, by BD (1 mentions) Cd3 145 2c11, by BD (1 mentions) Gr 1 rb6 8c5, by BD (1 mentions) C kit 2b8, by BD (1 mentions) Cd43 s7, by BD (1 mentions) Cd4 h129, by BD (1 mentions) B220 ra3 6b2, by BD (1 mentions) Accuri c6, by BD (1 mentions) Anti cd16 32 clone 93, by BioLegend (1 mentions)

4 protocols using cd25 7d4

1

Hematopoietic Cell Profiling in Mouse Tissues

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Hematopoietic stem and progenitor cell and lineage-positive cell profiling was perform on BM, spleen, and thymus of mice from each genotype. Antibodies (clones) used: CD3 (145-2C11) [BD: #553064], CD4 (H129.19) [BD: #553653], CD8 (53-6.7) [BD: #553033], Gr-1 (RB6-8C5) [BD: #553128], B220 (RA3-6B2) [BD: #553090], Ter119 (Ter119) [BD: #553673], Mac1 (M1/70) [BD: #553311], IL7Rα (A7R34) [eBiosceinces: #12-1271-82], cKit (2B8) [BD: #558163], Sca-1 (E13-161.7) [eBiosceinces: #17-5981-83], CD25 (7D4) [eBiosciences: 13-0252-82], CD44 (IM7) [BD: 559250], CD43 (S7) [BD: 561856], HSA (M1/69) [BD: #553262] and BP-1 (6C3) [BD: #553159], CD4 (GK1.5) [BD: #561830], CD8 (7D4) [eBiosciences: #12-0081-82], B220 (RA3-6B2) [BD: #561880].
Flow cytometric gating strategy is described in Supplementary Fig. 6.
Khattar E., Maung K.Z., Chew C.L., Ghosh A., Mok M.M., Lee P., Zhang J., Chor W.H., Cildir G., Wang C.Q., Mohd-Ismail N.K., Chin D.W., Lee S.C., Yang H., Shin Y.J., Nam D.H., Chen L., Kumar A.P., Deng L.W., Ikawa M., Gunaratne J., Osato M, & Tergaonkar V. (2019). Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy. Nature Communications, 10, 5349.
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2

Multiparameter Flow Cytometry Analysis

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Cells were stained using antibodies against surface markers CD3 (145-2C11), CD4 (RM4-5) and CD25 (7D4) (all from eBioscience or BD Biosciences), permeabilized, fixed and labeled for intracellular Foxp3 (FJK-16s), IL-4 (11B11), IFNγ (XMG1.2) and IL-17 (eBio17B7). Samples were acquired on LSRII flow cytometer with use of FACSDiva software (BD Biosciences). Data were analyzed using FlowJo software (TreeStar).
de Almeida Nagata D.E., Ting H.A., Cavassani K.A., Schaller M.A., Mukherjee S., Ptaschinski C., Kunkel S.L, & Lukacs N.W. (2015). Epigenetic control of Foxp3 by SMYD3 H3K4 histone methyltransferase controls iTreg development and regulates pathogenic T cell responses during pulmonary viral infection. Mucosal immunology, 8(5), 1131-1143.
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3

Quantification of Regulatory T Cells

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Mice were immunized i.n. with ova and NE (ova-NE) or non-adjuvated ova (ova-PBS) at weeks 0 and 4. Splenocytes were harvested at 1 and 7 weeks after the first immunization. Red blood cell depleted single cell suspensions were stained by flow cytometry to quantify regulatory T cells. Fc receptors were blocked with purified anti-CD16/32 (clone 93, BioLegend) and surface markers were stained using antibodies against CD3 (145-2C11), CD4 (RM4-5) and CD25 (7D4) (all from eBioscience or BD Biosciences), permeabilized, fixed and labeled for intracellular Foxp3 (FJK-16s). Samples were acquired on an Accuri C6 flow cytometer (BD Biosciences). Data were analyzed using FlowJo (Treestar).
Bielinska A.U., O’Konek J.J., Janczak K.W, & Baker JR J.r. (2016). Immunomodulation of TH2 biased immunity with mucosal administration of nanoemulsion adjuvant. Vaccine, 34(34), 4017-4024.
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4

Multiparameter Flow Cytometry Analysis

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Cells were stained using antibodies against surface markers CD3 (145-2C11), CD4 (RM4-5) and CD25 (7D4) (all from eBioscience or BD Biosciences), permeabilized, fixed and labeled for intracellular Foxp3 (FJK-16s), IL-4 (11B11), IFNγ (XMG1.2) and IL-17 (eBio17B7). Samples were acquired on LSRII flow cytometer with use of FACSDiva software (BD Biosciences). Data were analyzed using FlowJo software (TreeStar).
de Almeida Nagata D.E., Ting H.A., Cavassani K.A., Schaller M.A., Mukherjee S., Ptaschinski C., Kunkel S.L, & Lukacs N.W. (2015). Epigenetic control of Foxp3 by SMYD3 H3K4 histone methyltransferase controls iTreg development and regulates pathogenic T cell responses during pulmonary viral infection. Mucosal immunology, 8(5), 1131-1143.
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