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Ethylene diamine tetraacetic acid disodium edta na2

Manufactured by Merck Group
Sourced in Germany, United States

Ethylene diamine tetraacetic acid disodium (EDTA.Na2) is a chemical compound commonly used as a chelating agent in laboratory settings. It binds to metal ions, including calcium and magnesium, forming stable complexes. The disodium salt form is water-soluble and frequently utilized in various analytical and purification procedures.

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2 protocols using ethylene diamine tetraacetic acid disodium edta na2

1

Titration of Citric Acid Monohydrate

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Citric acid monohydrate (99.5%, MERCK, Darmstadt, Germany, AR grade) (CA), glycerol anhydrous (GLC) (98%, MERCK, Darmstadt, Germany, AR grade), ethylene diamine tetraacetic acid disodium (EDTA.Na2) (99.0%, Sigma-Aldrich, St. Louis, MO, USA), tetrahydrofuran (THF) (99.9%, MERCK, Darmstadt, Germany, AR grade), Eriochrome Black-T Indicator (EBT), and borax sodium hydroxide buffer solution were purchased from the Sigma-Aldrich Company (St. Louis, MO, USA). All chemicals were employed without further purification. Distilled and de-ionized water was used throughout all the experiments.
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2

Macrovipera lebetina obtusa Venom Assay

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The chemicals were from Sigma. The macrovipera lebetina obtusa (MLO) venom was purchased from Latoxan (Portes-lès-Valence, France). The bromphenacyl bromide (BPB) and ethylenediaminetetraacetic acid disodium (EDTA-Na2) were from Sigma-Aldrich (Saint Louis, MO, USA). The cell culture reagents and consumables were from Thermo Fisher Scientific (Waltham, MA, USA). The consumables used in the Seahorse XF assays were from Agilent Technologies (Santa Clara, CA, USA).
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