Human keratinocytes cells were treated with 5 µmoL of long chain ceramides, C18:0 and C 24:0 (Avanti) and collected 48 hours post-treatment. Nuclear protein was extracted using Nuclear Extraction Kit (RayBiotech) and PPARB trans-activity was measured using PPAR delta Transcription Factor Assay Kit (Abcam) as per manufacturer’s instructions. Details in Supplemental Information.
Ppar delta transcription factor assay kit
Manufactured by Abcam
The PPAR delta Transcription Factor Assay Kit is a quantitative assay kit designed to measure the activity of the PPAR delta transcription factor in nuclear extracts or whole cell lysates. The kit provides a quick and sensitive method to quantify PPAR delta activation or inhibition in a 96-well format.
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Lab products found in correlation
2 protocols using ppar delta transcription factor assay kit
1
Ceramide-Induced PPAR Delta Activity
2
PPAR delta Activation by Ceramides
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