Mycoseq detection kit

All PDAC cells were purchased from ATCC, which performs authentication on its own cell lines. Pa01c, Pa02c, Pa03c and Pa14c were kind gifts from Dr. Channing Der at UNC-CH and were whole-exome sequenced(11 (link)). HEK T/tH cells were a kind gift from Dr. Christopher Counter and previously described(12 (link)). KPC2 cells were a kind gift from Dr. David DeNardo (Washington University, MO), whole-exome sequenced and published (13 (link)). All cells were cultured in DMEM or RPMI plus 10% FBS/1% Pen-Strep at 5% CO₂ in 37°C incubators. Mycoplasma testing was performed every 6 months using MycoSEQ Detection kit (Applied Biosystems). All cell lines were used for fewer than 6 months after receipt or resuscitation from cryopreservation.

All human cell lines were purchased from ATCC, which performs its own authentication by short tandem repeat DNA profiling. Human CAF-1, CAF-2 and CAF-3 were isolated from surgical specimens of patients who underwent Whipple procedure, and expanded using published method(23 (link)). SC00A5 and CAF-8 were purchased from Vitro Biopharma, which performs its own authentication. All CAFs were confirmed to express α-SMA by western blots, as well as by immunofluorescence (IF) to express α-SMA but not pan-cytokeratin. KPC2 cells were a kind gift from Dr. David DeNardo (Washington University in St. Louis, WUSTL), were published and authenticated by whole-exome sequencing(24 (link)). All cells were cultured in DMEM/10% FBS/1% Pen-Strep at 5% CO₂, 37°C incubators. Mycoplasma testing was performed every 6 months using MycoSEQ Detection kit (Applied Biosystems). All lines were used for fewer than 6 months after receipt or resuscitation from cryopreservation.