Rabbit anti nf h

Manufactured by Merck Group

Sourced in United States

Rabbit anti-NF-H is a primary antibody that binds to the heavy chain of neurofilament proteins (NF-H) present in neuronal cells. It is used in various research applications to detect and study the distribution and expression of NF-H in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using rabbit anti nf h

Dorsal Root Ganglia Myelination Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dorsal root ganglia (DRG) were dissected from embryos at embryonic day 13.5 (E13.5) and plated singularly on collagen-coated coverslips as previously described [27 (link)]. The embryos were genotyped immediately after dissection. Myelination was induced with 50 μg/ml ascorbic acid (Sigma-Aldrich). Treatment with IFB-088 at the indicated concentration was applied for 2 weeks in parallel to the induction of myelination. Samples were then fixed, and rat anti-MBP (1/5) [28 (link)] and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4 °C. The following day, DRGs were washed and FITC- or TRITC-conjugated secondary antibodies (1:200, Cappel) were added for 1 h at room temperature. Specimen were incubated with DAPI (1:1000, SIGMA) and mounted with VECTASHIELD (Vector Laboratories). Eight to 10 images were taken from each DRG using a fluorescence microscope (Leica DM5000) with a 10 × objective, and the number of MBP + internodes in each image was counted.

Bai Y., Treins C., Volpi V.G., Scapin C., Ferri C., Mastrangelo R., Touvier T., Florio F., Bianchi F., Del Carro U., Baas F.F., Wang D., Miniou P., Guedat P., Shy M.E, & D’Antonio M. (2022). Treatment with IFB-088 Improves Neuropathy in CMT1A and CMT1B Mice. Molecular Neurobiology, 59(7), 4159-4178.

+ Open protocol

+ Expand

Visualization of Muscle Spindle Innervation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serial 90 μm sections were cut from fresh-frozen gastrocnemius dissected from six-week-old WT and Adv-KO-SCOT mice. Sections were blocked overnight in Superblock, 1.5% Normal Donkey Serum, 0.5% Porcine Gelatin, and 0.5% Triton X-100 at room temperature. Sections were incubated with mouse anti-myosin heavy chain, slow developmental (S46, 1:50; Developmental Studies Hybridoma Bank; Iowa City, IA) and rabbit anti-NF-H (1:500; EMD Millipore; Burlington, MA) for 24 hours at room temperature. Slides were washed twice with filtered PBS and then incubated with AlexaFluor 555-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes, 1:1000) secondary antibodies and Hoescht 33342 stain (Molecular Probes, 1:2000) for three hours at room temperature. Slides were washed again twice with filtered PBS. Slides were coverslipped and imaged with a Nikon Eclipse Ti2 inverted microscope at 40x. Two to seven muscle spindles were imaged per animal. For each spindle, the average width of three or more axonal rotations and the average distance between axonal rotations were measured by ImageJ.

Enders J., Jack J., Thomas S., Lynch P., Lasnier S., Cao X., Swanson M.T., Ryals J.M., Thyfault J.P., Puchalska P., Crawford P.A, & Wright D.E. (2023). Ketolysis is Required for the Proper Development and Function of the Somatosensory Nervous System. bioRxiv.

+ Open protocol

+ Expand

Immunofluorescent Staining of Motor Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Motor Neurons were fixed with 4% PFA for 10 min at room temperature. Permeabilization and blocking were done with 0.01% (w/v) Tween/PBS containing 1.5% BSA for 1 h. Cells were incubated overnight at 4°C in primary antibodies mouse anti-islet1/2 (1:1000, DSHB) and rabbit anti-NF-H (1:500, Millipore) were diluted in 0.01% Triton X-100/1x PBS. Cells were then washed 3 times with 1x PBS, and incubated with secondary antibodies (1:500) for 30 min-1h. After two subsequent washes with 1x PBS, cells were incubated with 1:5000 DAPI for 5 min, and stored in 1x PBS until imaging.

Pantazis C.B., Yang A., Lara E., McDonough J.A., Blauwendraat C., Peng L., Oguro H., Kanaujiya J., Zou J., Sebesta D., Pratt G., Cross E., Blockwick J., Buxton P., Kinner-Bibeau L., Medura C., Tompkins C., Hughes S., Santiana M., Faghri F., Nalls M.A., Vitale D., Ballard S., Qi Y.A., Ramos D.M., Anderson K.M., Stadler J., Narayan P., Papademetriou J., Reilly L., Nelson M.P., Aggarwal S., Rosen L.U., Kirwan P., Pisupati V., Coon S.L., Scholz S.W., Priebe T., Öttl M., Dong J., Meijer M., Janssen L.J., Lourenco V.S., van der Kant R., Crusius D., Paquet D., Raulin A.C., Bu G., Held A., Wainger B.J., Gabriele R.M., Casey J.M., Wray S., Abu-Bonsrah D., Parish C.L., Beccari M.S., Cleveland D.W., Li E., Rose I.V., Kampmann M., Aristoy C.C., Verstreken P., Heinrich L., Chen M.Y., Schüle B., Dou D., Holzbaur E.L., Zanellati M.C., Basundra R., Deshmukh M., Cohen S., Khanna R., Raman M., Nevin Z.S., Matia M., Van Lent J., Timmerman V., Conklin B.R., Chase K.J., Zhang K., Funes S., Bosco D.A., Erlebach L., Welzer M., Kronenberg-Versteeg D., Lyu G., Arenas E., Coccia E., Sarrafha L., Ahfeldt T., Marioni J.C., Skarnes W.C., Cookson M.R., Ward M.E, & Merkle F.T. (2022). A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell stem cell, 29(12), 1685-1702.e22.

+ Open protocol

+ Expand

Protein Analysis Techniques in Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

For western blot analysis and immunohistochemistry the following antibodies were used: goat anti-ChAT (Millipore); rat anti-LAMP1 (Iowa Hybridoma bank); Guinea pig anti-P62 (Progen); rabbit anti-LC3 (Sigma); rat anti-MBP (Chemicon); rabbit anti-NF-H (Millipore); mouse anti-TUJ1 (Promega); mouse anti-tubulin (Sigma); rabbit anti-actin (Sigma); mouse anti-FIG4 (Neuromab); rabbit anti-S100 (Sigma), rabbit anti-NGF receptor p75 (Millipore); chicken anti-P0 (Millipore) and mouse anti-P0 antibodies (kindly provided by J.J. Archelos); rabbit anti-ubiquitin (Santa Cruz); rhodamine phalloidin (Molecular Probes).
Protein lysates were prepared using a lysis buffer containing 1% Triton X-100 (or NP40 1% for autophagy marker evaluation), 50 mm Tris buffer, pH 8.0, 150 mm NaCl, 10 mm NaF, 1 mm Na vanadate, Complete (Roche) protease inhibitors.

Vaccari I., Carbone A., Previtali S.C., Mironova Y.A., Alberizzi V., Noseda R., Rivellini C., Bianchi F., Del Carro U., D'Antonio M., Lenk G.M., Wrabetz L., Giger R.J., Meisler M.H, & Bolino A. (2014). Loss of Fig4 in both Schwann cells and motor neurons contributes to CMT4J neuropathy. Human Molecular Genetics, 24(2), 383-396.

+ Open protocol

+ Expand

Immunophenotyping of Neural Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultured media, fetal bovine serum (FBS), serum-free supplements, and antibiotics were purchased from Invitrogen (Carlsbad, CA, United States). Tissue culture plastics were obtained from BD Bioscience (San Jose, CA, United States). Millicell culture inserts were purchased from Millipore (Watford, United Kingdom). Rabbit or mouse anti-bIII tubulin (clone TUJ-1, Covance, NJ, United States), rabbit anti-GFAP (Dako Cytomation, Ely, United Kingdom), mouse anti-5’-bromo-2’-deoxyuridine (BrdU; Millipore, Watford, United Kingdom), mouse anti-TH (Millipore, Darmstadt, Germany), goat anti-Iba1 (Abcam, MA, United States), rabbit anti-NFH (Millipore, Darmstadt, Germany), mouse anti-GFP (Millipore, Darmstadt, Germany), and goat anti-b-actin (Santa Cruz Biotechnology, TX, United States) primary antibodies were used. Unless stated otherwise, all other chemicals were purchased from Sigma-Aldrich Co (St. Louis, MO, United States).

Tsai M.J., Hung S.C., Weng C.F., Fan S.F., Liou D.Y., Huang W.C., Liu K.D, & Cheng H. (2021). Stem cell transplantation and/or adenoviral glial cell line-derived neurotrophic factor promote functional recovery in hemiparkinsonian rats. World Journal of Stem Cells, 13(1), 78-90.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!