Log In Sign up
The largest database of trusted experimental protocols

Crkl c20

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

CRKL (C20) is a primary antibody produced by Santa Cruz Biotechnology for the detection of CRKL protein in various samples. CRKL is a member of the CRK family of adapter proteins that play a role in signal transduction pathways.

Automatically generated - may contain errors

Lab products found in correlation

Paxillin, by BD (1 mentions) Phospho akt s473, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) Cd63 mx 49, by Santa Cruz Biotechnology (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) P130cas, by BD (1 mentions) Cabl k 12, by Santa Cruz Biotechnology (1 mentions) Phosphotyrosine 4g10, by Merck Group (1 mentions)

2 protocols using crkl c20

1

Immunoblot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblot analyses were carried out as described previously.32, 39 The detailed protocols are described in Appendix S1. Antibodies against the following proteins were purchased. ErbB3 (C‐17), DOCK180 (H4), C3G (C19), Gab1, CRKL (C20), and CD63 (MX‐49.129.5) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and paxillin, p130Cas, and CRK were from BD Transduction Laboratories (Lexington, KY, USA). Epidermal growth factor receptor (EGFR), phospho‐EGFR (Y1148), c‐Met, phospho‐c‐Met (Y1234/1235), ErbB2 (29D8), focal adhesion kinase (FAK), phospho‐FAK (Y397), phospho‐Gab1 (Y307), Src, phospho‐Src (Y416), ERK1/2, phospho‐ERK1/2, Akt, and phospho‐Akt (S473) were from Cell Signaling Technology (Beverly, MA, USA). Actin was from Chemicon International (Temecula, CA, USA) and α‐tubulin was from Sigma‐Aldrich.
Yoshida K., Tsuda M., Matsumoto R., Semba S., Wang L., Sugino H., Tanino M., Kondo T., Tanabe K, & Tanaka S. (2019). Exosomes containing ErbB2/CRK induce vascular growth in premetastatic niches and promote metastasis of bladder cancer. Cancer Science, 110(7), 2119-2132.
+ Open protocol
+ Expand
2

Western Blot Analysis of Phosphotyrosine Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
1.0 × 106 cells were treated for 4 h as indicated, lysed, and protein quantified as previously described [37 (link)]. Equal concentrations of protein were subjected to SDS-PAGE electrophoresis and protein transferred to PVDF membrane as previously described [37 (link)]. Antibodies to cAbl (K-12, Santa Cruz Biotechnology), Crk-L (C-20, Santa Cruz Biotechnology), and phosphotyrosine (4G10, Millipore) were used for Western blotting. All antibodies were used according to manufactures suggested protocols. Western blots were developed with horseradish peroxidase-conjugated goat anti-mouse IgG (heavy plus light chain, KPL) or goat anti-rabbit IgG (heavy plus light chain, KPL) and visualized using enhanced chemiluminescence and x-ray film.
Oaxaca D.M., Yang-Reid S.A., Ross J.A., Rodriguez G., Staniswalis J.G, & Kirken R.A. (2016). Sensitivity of imatinib-resistant T315I BCR-ABL CML to a synergistic combination of ponatinib and forskolin treatment. Tumour Biology, 37(9), 12643-12654.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!