Xpert mtb rif xpert assay

Manufactured by Cepheid

Sourced in United States

The Xpert MTB/RIF (Xpert) assay is a rapid molecular diagnostic test designed to detect Mycobacterium tuberculosis (MTB) and identify resistance to rifampicin (RIF) directly from a patient's sputum sample. The assay utilizes real-time PCR technology to provide qualitative results within a short timeframe.

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Xpert MTB/RIF (127 citations) Xpert MTB/RIF assay (66 citations) Xpert MTB/RIF Ultra assay (5 citations) Xpert MTB/RIF assay G4 version 5 (2 citations)

2 protocols using xpert mtb rif xpert assay

Xpert MTB/RIF Assay Optimization

Cited 1 time

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The Xpert MTB/RIF (Xpert) assay was performed according to the manufacturer's instructions (Cepheid). Four of the specimens were not examined. Two of the samples were found to contain non-tuberculosis mycobacteria by MALDI-TOF, and thus we skipped the Xpert experiments, because non-tuberculosis mycobacteria do not produce MPT64 [29 (link),30 (link)]. The other two samples were the same as those identified as different samples, and thus we also skipped the Xpert experiments for these samples. Furthermore, Xpert could not be applied when the sputum volume of the specimen was too low.

Wang W.H., Takeuchi R., Jain S.H., Jiang Y.H., Watanuki S., Ohtaki Y., Nakaishi K., Watabe S., Lu P.L, & Ito E. (2020). A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity. EBioMedicine, 60, 103007.

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Comprehensive Tuberculosis Evaluation

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A pus specimen collected during the operation from each patient was subjected to smear microscopy, culture, and Xpert MTB/RIF (Xpert) assay (Cepheid, Sunnyvale USA), while a tissue specimen was subjected to histopathology examination, simultaneously. Drug susceptibility testing (DST) was conducted for all the recovered isolates.

Fan J., Lan T., Tang K., Wang G., Dong W., Li D., Yan G., Li Y., Lei G., Shan J., Wang Q., Ma J., Huang H, & Qin S. (2021). The Comparative Influence of 2 and 4 Weeks Preoperative Antituberculosis Treatment on Spinal Tuberculosis Surgery: A Multicenter, Prospective, Randomized Clinical Trial. Infectious Diseases and Therapy, 10(3), 1451-1463.

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