Immpact amec red peroxidase hrp substrate

Manufactured by Vector Laboratories

Sourced in United States

ImmPACT AMEC Red Peroxidase (HRP) Substrate is a chromogenic substrate used for the detection and visualization of horseradish peroxidase (HRP) in immunohistochemical and other assays. It produces a red-brown colored precipitate at the site of the HRP enzyme.

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Lab products found in correlation

Acta2, by Cell Signaling Technology (1 mentions) Dp70 digital camera, by Olympus (1 mentions) Bh 2 microscope, by Olympus (1 mentions) Anti lamp1 antibody, by Cell Signaling Technology (1 mentions) Accustain trichrome stain kit, by Merck Group (1 mentions) Vectastain elite abc hrp kit, by Vector Laboratories (1 mentions) Oct compound, by Sakura Finetek (1 mentions)

Close spelling variants of this product

ImmPACT AMEC Red Peroxidase Substrate Immpact AMEC red

4 protocols using immpact amec red peroxidase hrp substrate

Immunohistochemistry with ImmPACT AMEC Red

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Immunohistochemistry was performed according to the method previously described in [8 (link)], except that the signal was detected using an ImmPACT AMEC Red Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA, USA), according to the manufacturer’s instructions.

Ogiwara K, & Takahashi T. (2019). Nuclear Progestin Receptor Phosphorylation by Cdk9 Is Required for the Expression of Mmp15, a Protease Indispensable for Ovulation in Medaka. Cells, 8(3), 215.

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Immunohistochemical Analysis of Kidney Vasculature

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Kidney sections were stained with rat anti-cluster of differentiation 31 (CD31, Dianova, Hamburg, Germany) and rabbit anti-smooth muscle actin alpha 2 (ACTA2, Cell Signaling, Danvers, MA, USA) to determine co-localization of endothelial and myofibroblasts markers. ImPRESS-AP (alkaline phosphatase) polymer anti-rat IgG and ImPRESS-HRP anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA), were used as secondary antibodies. Highdef Blue IHC chromogen AP (Enzo, Farmingdale, NY, USA) and ImmPACT AMEC Red peroxidase HRP substrate (Vector Laboratories) were used for developing. Microscopy images were acquired on an Olympus BH-2 microscope, and captured with an attached Olympus 12.5 million pixel, DP70 Digital Camera. Images were analyzed blinded to group identity by two independent investigators.

Valero-Muñoz M., Oh A., Faudoa E., Bretón-Romero R., El Adili F., Bujor A, & Sam F. (2021). Endothelial-mesenchymal transition in HFpEF: insights into the cardiorenal syndrome. Circulation. Heart failure, 14(9), e008372.

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Quantification of LAMP1 in HAECs

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HAECs were exposed to either control or excess nutrient conditions as described above and fixed in 0.4% formalin. Antigen retrieval was performed using citrate buffer. In conjunction with anti-LAMP1 antibody (Cell Signaling Technologies) Ultratech HRP 500–600 tests (catalogue # IM2391, Beckman-Coulter) and ImmPACT AMEC Red Peroxidase HRP substrate (catalogue # SK-4285, Vector Laboratories) were used to visualize LAMP1. ImageJ (NIH) was used to quantify LAMP1 staining relative to the number of cells in each image.

Weikel K.A., Cacicedo J.M., Ruderman N.B, & Ido Y. (2016). Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells. Bioscience Reports, 36(5), e00382.

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Cardiac Tissue Immunohistochemistry and Trichrome Staining

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For immunohistochemistry, hearts were harvested and embedded in O.C.T compound (Sakura Finetek). Embedded tissues were snap-frozen in dry ice. Sections of 5 µm thickness were then stained using antibodies directed against CD11b (clone M1/70) or CD31 (clone MEC 13.3) (all BioRad). Staining was followed with a biotinylated secondary antibody. We used the VECTASTAIN Elite ABC HRP kit and ImmPACT AMEC Red Peroxidase (HRP) substrate (Vector Laboratories, Inc.) for color development. For Masson’s trichrome staining, we used Weigert’s iron hematoxylin solution and Accustain Trichrome Stain Kit (both Sigma-Aldrich) according to the manufacturer’s protocol.

Seung H., Wrobel J., Wadle C., Bühler T., Chiang D., Rettkowski J., Cabezas-Wallscheid N., Hechler B., Stachon P., Maier A., Weber C., Wolf D., Duerschmied D., Idzko M., Bode C., von zur Mühlen C., Hilgendorf I, & Heidt T. (2022). P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Research in Cardiology, 117(1), 16.

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