Media for each cell line

CdCl₂, sterile dimethylsulfoxide, sterile phosphate-buffered saline, and molecular grade water were purchased as the highest grade required from Sigma-Aldrich (St. Louis, MO USA), Fisher Scientific (Houston, TX USA), and Pierce Biotechnology (Rockford, IL USA). ELISA kits were obtained from Pierce Biotechnology (Rockford, IL USA). Antibodies were obtained from R&D Systems Inc., (Minneapolis, MN USA), Cell Signaling Technology, Inc. (Danvers, MA USA), and Thermo-Fisher Scientific (Waltham, MA USA). Media for each cell line were obtained from Corning Life Sciences (Tewksbury, MA USA). Media supplements were purchased from Sigma-Aldrich (St. Louis, MO USA), and these included penicillin/streptomycin, glucose, glutamine, and sodium bicarbonate. Foetal bovine serum, triple 0.1 μm filtered, was purchased from Atlanta Biologicals through R&D Systems (Minneapolis, MN USA).

Media for each cell line was purchased from Corning Life Sciences (Tewksbury, MA, USA). Media supplements were obtained from Sigma-Aldrich (St. Louis, MO, USA), including penicillin/streptomycin, glucose, glutamine, sodium bicarbonate. FBS, triple-0.1-micrometer-filtered, was purchased from Atlanta Biologicals through R&D Systems (Minneapolis, MN, USA). NiCl₂, Ni-acetate, sterile dimethylsulfoxide (DMSO). Sterile phosphate-buffered saline (PBS) and molecular-grade water were purchased from Sigma-Aldrich (St. Louis, MO, USA), Fisher Scientific (Houston, TX, USA), and Pierce Biotechnology (Rockford, IL, USA). ELISA kits were obtained from Pierce Biotechnology (Rockford, IL, USA), while antibodies were purchased from R&D Systems Inc., (Minneapolis, MN, USA), Cell Signaling Technology, Inc. (Danvers, MA, USA), and Thermo Fisher Scientific (Waltham, MA, USA).