Dichloroacetonitrile

Bromoacetonitrile (Sigma Aldrich, 242489), diBromoacetonitrile (Alfa Aesar, A16994), chloroacetonitrile (Sigma Aldrich, C19651), dichloroacetonitrile (Alfa Aesar, A10612), trichloroacetonitrile (Sigma Aldrich, T53805), phostag-acrylamide (AlphaLabs, AAL-107, 300-93523), hydroxyurea (Formedium, HDU0025).

Chloroacetamide (CAcAm) (98.5%), DCAcAm (98.5%), and TCAcAm (99%) standards were obtained from Alfa Aesar (Karlsruhe, Germany). Bromochloro- (BCAcAm), dibromo- (DBAcAm), bromodichloro- (BDCAcAm), dibromochloro- (DBCAcAm), and tribromoacetamide (TBAcAm) standards were all purchased from Orchid Cellmark (New Westminster, BC, Canada). Bromoacetamide (BAcAm), two haloacetonitriles (HANs) (dichloroacetonitrile [DCAN] and trichloroacetonitrile [TCAN]), and the model compounds (Tyr [≥99%], Ala [≥99%], Tyr-Ala [>98%], Ala-Tyr [>98%], Ala-Ala [>98%], Tyr-Tyr-Tyr [>98%], and Ala-Ala-Ala [>98%]) were purchased from Sigma–Aldrich (Oakville, ON, Canada). A sodium hypochlorite solution (reagent grade [>98%], active chlorine >5%, Sinopharm Chemical Reagent Co., Ltd., China) was used to prepare free chlorine stock solutions. The ultrapure water was produced with a Millipore Milli-Q Gradient water purification system (Billerica, MA, USA). All the other chemical reagents were at least of analytical grade, and obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) unless otherwise noted.