Sprague dawley cd

All animal protocols were approved by the Institutional Animal Care and Use Committee at the University of Colorado at Boulder. Rats were fed ad libitum standard rodent chow and housed in a facility with a 12 hour light, 12 hour dark cycle. Male and female (300–400g, 3–4 months old) Sprague-Dawley, CD, rats were purchased from Charles River Laboratory and acclimated to the facility prior to the described experiments. The females were not staged by estrous cycle for the following experiments. For the ovariectomy experiment, 3-month-old female Sprague Dawley, CD, rats that underwent either a sham or ovariectomy (OVX) procedure were purchased from Charles River Laboratory. Cardiac myocytes were isolated from sham and OVX animals three weeks after surgery.

Animals were handled and housed according to the National Institutes of Health Committee on Laboratory Animal Resources guidelines. Mice used were the C57BL/6J strain (The Jackson Laboratory); rats were Sprague-Dawley CD (Charles River). All experimental protocols (protocol #20160084) were approved by the Washington University in St Louis Institutional Animal Care and Use Committee. The animals were euthanized by carbon dioxide exposure, followed by cervical dislocation. Anesthesia was not used and every effort was made to minimize pain and discomfort.