Sodium bicarbonate

Manufactured by Vetec

Sourced in Brazil

Sodium bicarbonate is a white, crystalline powder. It has the chemical formula NaHCO3 and is commonly known as baking soda. The primary function of sodium bicarbonate is to act as a pH regulator, neutralizing acidic substances.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) L glutamine, by Vetec (2 mentions) Magnesium chloride hexahydrate, by Vetec (2 mentions) Carbamylcholine chloride, by Merck Group (2 mentions) Calcium chloride dihydrate, by Vetec (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Amphotericin b, by Merck Group (1 mentions) Acetylcholine ach, by Merck Group (1 mentions) Phenylephrine phe, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Benzoyl peroxide, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Vetec (1 mentions) Ketoconazole, by Vetec (1 mentions) Acetic anhydride, by Vetec (1 mentions) Methyl methacrylate, by Merck Group (1 mentions) Ethyl iodide, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Vetec (1 mentions)

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Sodium bicarbonate (NaHCO3) (3 citations)

6 protocols using sodium bicarbonate

Maintaining Cell Lines for Multidrug Resistance

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Cells and culture procedures
The K562, Lucena-1 and FEPS cell lines were provided of Immunology Laboratory at the Medical Biochemistry Institute Leopoldo de Meis of the Federal University of Rio de Janeiro (Brazil). The cells were were cultured in the Cell Culture Laboratory of the Federal University of Rio Grande at 37 • C in 5% CO 2 in disposable plastic flasks containing RPMI 1640 (Gibco, Brazil), medium supplemented with sodium bicarbonate (2.0 g/L) (Vetec, Brazil), l-glutamine (0.3 g/L) (Vetec, Brazil), Hepes (25 mM) (Acros, Belgium), 10% fetal bovine serum (Gibco, Brazil), 1% antibiotic (penicillin, 100 U/mL and streptomycin 100 mg/mL) (Gibco, Brazil) and antimycotic (amphotericin B 0.25 mg/mL -Sigma, Brazil). For maneitance of MDR phenotype, Lucena-1 cell line was maintained with 60 nM of VCR (Sigma, Brazil) and FEPS cell line with 300 ng/ml of DNR (Sigma, Brazil).

Marques M.B., Andrade F.R.T., Silva E.F.E., Oliveira B.R., Almeida D.V., de Souza Votto A.P, & Marins L.F. (2023). Effects of chemotherapeutic drugs on the antioxidant capacity of human erythroleukemia cells with MDR phenotype. Molecular and cellular biochemistry, 478(11).

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Rat Aortic Vascular Reactivity

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Calcium chloride dihydrate (CaCl₂.2H₂O), magnesium chloride
hexahydrate (MgCl₂.6H₂O), potassium chloride (KCl), and sodium
bicarbonate (NaHCO₃) were purchased from VETEC (Brazil). Monosodium
phosphate 1-hydrate (NaH₂PO₄.H₂O), glucose
(C₆H₁₂O₆), magnesium sulfate monohydrate
(MgSO₄.H₂O), and hydrochloric acid (HCl PA) were purchased
from Nuclear (Brazil). Sodium chloride (NaCl) was purchased from Dinâmica (Brazil).
Carbamylcholine chloride, acetylcholine (ACh), and phenylephrine (Phe) were purchased
from Sigma-Aldrich (USA). The carbogenic mixture (95% O₂ and 5%
CO₂) was purchased from White Martins (Brazil).

Brito A.F., Silva A.S., Souza I.L., Pereira J.C, & da Silva B.A. (2015). Intensity of swimming exercise influences aortic reactivity in rats. Brazilian Journal of Medical and Biological Research, 48(11), 996-1003.

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Ketoconazole-Loaded Polymeric Films

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The reagents used for the films and incorporation with ketoconazole were sodium hydroxide (NaOH), acetic anhydride (CH₃COOCOCH₃), acetic acid (CH₃COOH), hydrochloric acid (HCl), sodium bicarbonate (NaHCO₃), sulfuric acid (H₂SO₄), commercial ketoconazole, and dichloromethane (CH₂Cl₂), purchased from Vetec. Poly (vinyl alcohol) (average mol wt 30,000–70,000), ethyl iodide (CH₂CH₃I), chloride sodium (NaCl), benzoyl peroxide, methyl methacrylate (MMA), and distilled water were purchased from Sigma Aldrich. The copolymers PMMA-g-PEG 4000 and derivatives were synthesized by our research group and published in scientific journals [19 (link),20 (link),21 (link),22 (link),23 (link)].

Reynaldo J.R., Novack K.M., Sousa L.R., Vieira P.M., Amparo T.R., de Souza G.H., Teixeira L.F., Barboza A.P., Neves B.R., Alvarenga M.E., Martins F.T, & dos Santos V.M. (2022). Evaluation of Anti-Candida albicans Activity and Release of Ketoconazole in PMMA-G-PEG 4000 Films. International Journal of Molecular Sciences, 23(18), 10775.

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Pharmacological Evaluation of Compound Mixture

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Magnesium sulfate (MgSO₄), potassium chloride (KCl), calcium chloride (CaCl₂), glucose, sodium bicarbonate (NaHCO₃), sodium chloride (NaCl), and sodium dihydrogen phosphate (NaH₂PO₄) were purchased from Vetec Química Fina Ltda. (Brazil). Atropine (99%), (S-(−)-Bay K8644), chloride verapamil, pyrilamine, carbamylcholine hydrochloride (CCh), Cremophor®, and histamine were obtained from Sigma-Aldrich (Brazil). Carboxymethylcellulose was obtained from Formula (Brazil). Castor oil was obtained from Farmax (Brazil). Loperamide (99%) was obtained from Janssen Cilag Farmacêutica Ltd. (Brazil) and activated charcoal was obtained from Proquímicos (Brazil). All substances were diluted in distilled water, and the extract was solubilized in Cremophor^® (3%), whose concentration never exceeded 0.01% (v/v). At this concentration, this chemical is devoid of contractile or relaxant effects on the studied organ, according to previous obtained data (data not shown).

Pereira J.C., Figueiredo I.A., de Oliveira F.R., Ferreira S.R., Aires Moreno G.T., da Silva T.M., Pinheiro U.D., Santos B.V., da Silva B.A, & Cavalcante F.D. (2020). Oceanapia magna Sponge Presents Dual Effect on the Gastrointestinal Motility of Rodents: In Vitro and In Vivo Assays. Frontiers in Pharmacology, 11, 572574.

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Cytotoxicity of Microcystins in HTC Cells

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The HTC cell line was obtained from the Cell Bank of Rio de Janeiro, Brazil. HTC cells were grown in RPMI 1640 medium (Gibco, USA) supplemented with sodium bicarbonate (0.2 g/L) (Vetec, Brazil), L-glutamine (0.3 g/L) (Vetec, Brazil), Hepes (25 mM) (Acros, USA) and b-mercaptoethanol (5 × 10–5 M) (Sigma, Germany), with 10 % fetal bovine serum (Gibco, Brazil), 1 % of antibiotic and antimycotic (penicillin – 100 U/mL, streptomycin-100 mg/mL and amphotericin B - 0.25 mg/mL), in disposable plastic flasks, at 37°C.
The cytotoxicity assay was performed by trypan blue exclusion after 24 hours of incubation with microcystins. Three independent experiments were carried out using triplicates in each experiment. Data are expressed as mean + standard error and analyzed using ANOVA followed by Tukey’s multiple range test. Significance level was fixed in 0.05.

Ramos D.F., Matthiensen A., Colvara W., de Votto A.P., Trindade G.S., da Silva P.E, & Yunes J.S. (2015). Antimycobacterial and cytotoxicity activity of microcystins. The Journal of Venomous Animals and Toxins Including Tropical Diseases, 21, 9.

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Preparation of Physiological Solutions

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Calcium chloride dihydrate (CaCl₂.2H₂O), magnesium chloride
hexahydrate (MgCl₂.6H₂O), potassium chloride (KCl) and sodium
bicarbonate (NaHCO₃) were purchased from VETEC (Brazil). Monosodium phosphate
1-hydrate (NaH₂PO₄.H₂O), glucose
(C₆H₁₂O₆), magnesium sulfate monohydrate
(MgSO₄.H₂O) and hydrochloric acid (HCl) were purchased from
Nuclear (Brazil). Sodium chloride (NaCl) was purchased from Dinâmica (Brazil).
Carbamylcholine chloride (CCh), aminophylline, trichloroacetic acid, arachidonic acid
(AA), and thiobarbituric acid were purchased from Sigma-Aldrich (Brazil). Carbogenic
mixture (95% O₂ and 5% CO₂) was purchased from White Martins
(Brazil).

Brito A.F., Silva A.S., Souza I.L., Pereira J.C., Martins I.R, & Silva B.A. (2015). Intensity of swimming exercise influences tracheal reactivity in rats. Journal of Smooth Muscle Research, 51, 70-81.

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