L norepinephrine

The following are the relevant ethylamines: 2-(4-fluorophenyl)ethylamine (1a), 2-(4-chlorophenyl)ethylamine (1b), 2-(2,4-dichlorophenyl)ethylamine (1c), 2-(4-bromophenyl)ethylamine (1d), 2-(4-methoxyphenyl)ethylamine (1e), 2-(3,4-dimethoxyphenyl)ethylamine (1f). The target analytes are as follows: tyramine; L-norepinephrine or 3,4-dihydroxyphenylacetic acid; and methacrylic acid, methacryloyl chloride, divinylbenzene were sourced from Sigma-Aldrich (Steinheim, Germany). The polymerization reaction initiator, 1,1′-azobiscyclohexanecarbonitrile, was from Merck (Darmstadt, Germany). The relevant solvents (ethylene dichloride, hexane, petroleum ether, toluene, methanol, hydrochloric acid (36%), triethylamine, and salts used in the synthesis and the post-polymerization treatment) were delivered from POCh (Gliwice, Poland). Ultra-pure water delivered from a Milli-Q purification system (Millipore, France) was used to prepare water solutions.

Amphotericin B (25 mg/mL stock in DMSO Sigma-Aldrich, cat. no. A2411) was used for inhibition of fungal growth. l-norepinephrine (10 mM stock solution in DMSO; Sigma-Aldrich, cat. no. A7257), acetylcholine chloride (10 mM stock in DMSO, Sigma-Aldrich, cat. no. A2661), fMLF (10 mM stock in DMSO, Sigma-Aldrich, cat. no. 47729), fMIFL (10 mM stock in DMSO, Phoenix Pharmaceuticals, cat. no. 072-05), tBOC (2 mM stock solution in DMSO, Tocris, cat. no. 3730) and human serum (Sigma-Aldrich, cat. no. H4522) were directly added to FPS_SEAP-engineered cells.

Oleic acid-capped quantum dots (QDs-OA) with alloyed (CdSe_xS_1-x) core and ZnS shell (CdSeS/ZnS), nanocrystal diameter = 6.0 nm, λ_em = 630 nm, 1 mg/mL in toluene were purchased from Cytodiagnostics Inc. (Burlington, Canada). Glutathione in reduced form (GSH) was from Acros Organics (Geel, Belgium). Tetramethylammonium hydroxide pentahydrate (TMAH) was from Alfa Aesar (Kandel, Germany). Chloroform, anhydrous ethanol, and phosphoric acid (85%) were obtained from Chempur (Piekary Śląskie, Poland). Dialysis tubing cellulose membrane (MWCO = 12 kDa), dopamine hydrochloride, L-epinephrine, L-norepinephrine, serotonin hydrochloride, γ-aminobutyric acid (GABA), acetylcholine chloride, sodium phosphate monohydrate, disodium phosphate dodecahydrate, and sodium hydroxide were supplied by Sigma-Merck (Poznań, Poland). Milli-Q water was used for preparation of all aqueous solutions. All chemicals were used as received.