Ab264052

Manufactured by Abcam

Sourced in United States

Ab264052 is a laboratory equipment product offered by Abcam. It serves as a core function for specific applications, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab55271, by Abcam (1 mentions) Sc 150, by Santa Cruz Biotechnology (1 mentions) Ab1791, by Abcam (1 mentions) Phospho mlkl ser358, by Cell Signaling Technology (1 mentions) H3k4me3, by Cell Signaling Technology (1 mentions) Sytox green, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab264052

Western Blot Analysis of Cellular Proteins

Cited 1 time

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Proteins were analyzed by Western Blot as previously described [13 (link)]. List of antibodies used in this work: anti-CEBPα (2841, CST, 1:1000), anti-CEBPβ (sc-150, Santa Cruz, 1:1000), anti-GAPDH (5174, CST, 1:10,000), anti-HSP90 (4874, CST, 1:1000), anti-IDH2 (ab55271, Abcam, 1:1000), anti-IDH2-R172K (ab264052, Abcam, 1:1000), anti-RARα (gifted by Prof. Hugues de Thé), anti-RXRα (3085, CST), anti-Actinin (3134, CST, 1:10,000), anti-VDR (12550, CST).

Sabatier M., Boet E., Zaghdoudi S., Guiraud N., Hucteau A., Polley N., Cognet G., Saland E., Lauture L., Farge T., Sahal A., Pancaldi V., Chu-Van E., Castelli F., Bertoli S., Bories P., Récher C., Boutzen H., Mansat-De Mas V., Stuani L, & Sarry J.E. (2021). Activation of Vitamin D Receptor Pathway Enhances Differentiating Capacity in Acute Myeloid Leukemia with Isocitrate Dehydrogenase Mutations. Cancers, 13(20), 5243.

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Analyzing Necroptosis Regulatory Proteins

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Nec-1 (HY-15760), Z-VAD(OMe)-FMK (HY-16658), Fer-1 (HY-100579), VX-765(HY-13205), 3-MA (HY-19312), Dox (HY-N0565A), AZA (HY-10586), and DEC (HY-A0004) were obtained from MedChem Express (Shanghai, China). SYTOX Green (S7020) was purchased from Life Technologies (CA, USA). R-2HG (SML2200) was from Sigma-Aldrich (Munich, German). Antibodies against RIPK1 (#3493), phospho-RIPK1 (Ser¹⁶⁶) (#65746), phospho-MLKL (Ser³⁵⁸) (#91689), MLKL (#14993), RIPK3 (#13526), phospho-RIPK3 (Ser²²⁷) (#93654), and H3K4me3 (#9751) were purchased from Cell Signaling Technology (MA, USA). Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#60004-1-Ig), FLAG (#66008-4-Ig), and IDH2 (#66918-1-Ig) were obtained from Proteintech Group (Wuhan, China). The KDM2B antibody (#17-10264) was purchased from Sigma-Aldrich (Munich, German). Antibodies against IDH2R172K (ab264052) and H3 (ab1791) were obtained from Abcam (MA, USA).

Zhu S., Luo Y., Li K., Mei C., Wang Y., Jiang L., Wang W., Zhang Q., Yang W., Lang W., Zhou X., Wang L., Ren Y., Ma L., Ye L., Huang X., Chen J., Sun J, & Tong H. (2024). RIPK3 deficiency blocks R-2-hydroxyglutarate–induced necroptosis in IDH-mutated AML cells. Science Advances, 10(16), eadi1782.

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