Immunoblotting was performed as described previously48 (link) with samples resolved on 4-12% Ready Gel precise Gels (BioRad, Hercules, CA), transferred onto PVDF membranes, and probed using primary antibodies: anti-phosphorylated FAK (Millipore, Billerica, MA), anti-FAK (Cell Signaling, Danvers, MA), and anti-SUSD3. Equal loading was confirmed using anti-β-actin (Sigma Aldrich, St. Louis, MO). Anti-mouse and rabbit IgG secondary antibodies were used (Cell Signaling). Western blots were developed using Amersham ECL Plus (GE Healthcare, San Francisco, CA) and SuperSignal West Femto Chemiluminescent substrate (Thermo Scientific). Quantification was performed using Image J (NIH, Bethesda, MD).
Anti phosphorylated fak
Manufactured by Merck Group
Anti-phosphorylated FAK is a lab equipment product that detects and quantifies the phosphorylated form of the Focal Adhesion Kinase (FAK) protein. FAK is a key signaling molecule involved in cellular processes such as cell adhesion, migration, and survival. The anti-phosphorylated FAK product allows researchers to study the activation and regulation of FAK in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (2 mentions)
Supersignal west femto chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Amersham ecl plus, by GE Healthcare (2 mentions)
Anti fak, by Cell Signaling Technology (2 mentions)
Ready gel precise gels, by Bio-Rad (2 mentions)
Amersham ecl, by Cytiva (2 mentions)
2 protocols using anti phosphorylated fak
1
Immunoblotting Analysis of FAK Phosphorylation
2
Immunoblotting Analysis of FAK Phosphorylation
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Immunoblotting was performed as described previously48 (link) with samples resolved on 4-12% Ready Gel precise Gels (BioRad, Hercules, CA), transferred onto PVDF membranes, and probed using primary antibodies: anti-phosphorylated FAK (Millipore, Billerica, MA), anti-FAK (Cell Signaling, Danvers, MA), and anti-SUSD3. Equal loading was confirmed using anti-β-actin (Sigma Aldrich, St. Louis, MO). Anti-mouse and rabbit IgG secondary antibodies were used (Cell Signaling). Western blots were developed using Amersham ECL Plus (GE Healthcare, San Francisco, CA) and SuperSignal West Femto Chemiluminescent substrate (Thermo Scientific). Quantification was performed using Image J (NIH, Bethesda, MD).
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