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Chmp4b myc

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CHMP4B-myc is a recombinant protein that contains the CHMP4B protein sequence tagged with a myc epitope. CHMP4B is a member of the charged multivesicular body protein (CHMP) family, which is involved in the formation of multivesicular bodies and the endosomal sorting complex required for transport (ESCRT) pathway.

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Lab products found in correlation

Mlv gag yfp, by Addgene (2 mentions) Mcherry halix, by Addgene (2 mentions)

2 protocols using chmp4b myc

1

Retroviral Infection of Mammalian Cells

Cited 1 time
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MLV-Gag-YFP (Plasmid 1813) and mCherry-hAlix (Plasmid 21504) constructs were obtained from Addgene51 (link), 52 (link). CHMP4B-myc was purchased from Origene (RC207637). Other cDNAs used in this study were previously described11 (link) and have been deposited with Addgene. For retroviral infection, VSV-G pseudotyped retroviruses were generated, and MEFs were infected and selected as previously described11 (link). Following selection, stable pools were used at a maximum of 6–8 passages to avoid clonal selection or drift.
Murrow L., Malhotra R, & Debnath J. (2015). ATG12-ATG3 Interacts with Alix to Promote Basal Autophagic Flux and Late Endosome Function. Nature cell biology, 17(3), 300-310.
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2

Retroviral Infection of Mammalian Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
MLV-Gag-YFP (Plasmid 1813) and mCherry-hAlix (Plasmid 21504) constructs were obtained from Addgene51 (link), 52 (link). CHMP4B-myc was purchased from Origene (RC207637). Other cDNAs used in this study were previously described11 (link) and have been deposited with Addgene. For retroviral infection, VSV-G pseudotyped retroviruses were generated, and MEFs were infected and selected as previously described11 (link). Following selection, stable pools were used at a maximum of 6–8 passages to avoid clonal selection or drift.
Murrow L., Malhotra R, & Debnath J. (2015). ATG12-ATG3 Interacts with Alix to Promote Basal Autophagic Flux and Late Endosome Function. Nature cell biology, 17(3), 300-310.
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+ Expand

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