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Linear polystyrene standards

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Linear polystyrene standards are reference materials used to calibrate and verify the performance of analytical instruments, such as gel permeation chromatography (GPC) or size exclusion chromatography (SEC) systems. These standards provide a well-defined set of molecular weights for polystyrene, which is a common polymer used in various applications.

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Lab products found in correlation

Cdcl3, by Bruker (2 mentions) Seveneasy ph meter, by Mettler Toledo (1 mentions) Talos l120c microscope, by Thermo Fisher Scientific (1 mentions) Zetasizer pro, by Malvern Panalytical (1 mentions)

4 protocols using linear polystyrene standards

1

Polymer Characterization by NMR and GPC

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Polymer structure was characterized by nuclear magnetic resonance spectroscopy (NMR) via 1H NMR in CDCl3 (Bruker 500 MHz) and analyzed using TopSpin 3.5 software. To measure polymer molecular weight and polydispersity, polymers were dissolved in BHT-stabilized tetrahydrofuran with 5% DMSO and 1% piperidine, filtered through a 0.2 μm PTFE filter, and measured with gel permeation chromatography against linear polystyrene standards (Waters, Milford, MA).
Rui Y., Wilson D.R., Sanders K, & Green J.J. (2019). Reducible branched ester-amine quadpolymers (rBEAQs) co-delivering plasmid DNA and RNA oligonucleotides enable CRISPR/Cas9 genome editing. ACS applied materials & interfaces, 11(11), 10472-10480.
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2

Polymer Characterization by NMR, GPC, and pH Titration

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Polymer structure was characterized by nuclear magnetic resonance (NMR) spectroscopy via 1H NMR in CDCl3 (Bruker 500 MHz) and analyzed using TopSpin 3.5 software. Polymer molecular weight was characterized by gel permeation chromatography (GPC); polymers were dissolved in butylated hydroxytoluene–stabilized tetrahydrofuran with 5% DMSO and 1% piperidine, filtered through a 0.2-μm polytetrafluoroethylene filter, and characterized using GPC against linear polystyrene standards (Waters, Milford, MA). pH titrations were performed using a SevenEasy pH meter (Mettler Toledo) with 10 mg of polymer dissolved in 10 ml of 100 mM NaCl acidified with HCl, as previously described (15 (link)). Polymer was titrated from pH 3.0 to 11.0 using 100 mM NaOH added stepwise, and pH was recorded after each addition.
Rui Y., Wilson D.R., Choi J., Varanasi M., Sanders K., Karlsson J., Lim M, & Green J.J. (2019). Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing. Science Advances, 5(12), eaay3255.
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3

GPC Characterization of Polymer MW

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Polymer molecular weight was characterized using gel permeation chromatography (GPC) against linear polystyrene standards (Waters, Milford, MA). Polymers were dissolved in butylated hydroxytoluene (BHT)-stabilized THF and filtered through 0.2-μm polytetrafluoroethylene (PTFE) filters before GPC measurements. Predicted polymer LogP values were calculated using the online cheminformatics software molinspiration.com.
Rui Y., Wilson D.R., Tzeng S.Y., Yamagata H.M., Sudhakar D., Conge M., Berlinicke C.A., Zack D.J., Tuesca A, & Green J.J. (2022). High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA. Science Advances, 8(1), eabk2855.
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4

Polymer NP Characterization by GPC, DLS, and TEM

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Polymer molecular weight was measured using GPC relative to linear polystyrene standards (Waters). The hydrodynamic diameter of the NPs in 1× PBS was measured by DLS using a Zetasizer Pro (Malvern Panalytical). Zeta potential was measured via electrophoretic mobility using the same instrument to characterize the surface charge of the NPs. NP size and morphology were visualized by TEM using a Talos L120C microscope (Thermo Scientific).
Ben-Akiva E., Karlsson J., Hemmati S., Yu H., Tzeng S.Y., Pardoll D.M, & Green J.J. (2023). Biodegradable lipophilic polymeric mRNA nanoparticles for ligand-free targeting of splenic dendritic cells for cancer vaccination. Proceedings of the National Academy of Sciences of the United States of America, 120(26), e2301606120.
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