Model pal bx acid f5

Respiratory rate was measured by using the infrared gas analyzer (Li-6262 CO₂/H₂O analyzer, LI-COR, Inc., USA) according to the method described by Wu et al. [5 (link)]. Twelve fruit of each replicate was randomly selected, weighted and placed in a 9.5 L plastic container. The change of CO₂ concentration was determined and the respiratory rate was expressed as CO₂ (mg kg⁻¹ h⁻¹, fresh weight). TSS and TA were determined by using a hand-held digital refractometer (Model PAL-BX|ACID F5, Atago, Tokyo, Japan) according to the manufacturer’s instructions. TSS was expressed as Brix (%). A total of five biological replicates of 0, 1, 4, and 7 d samples were used to the measure respiratory rate, TSS and TA.

TSS and TA of the fruit juice were measured by a Brix-Acidity Meter (Model PAL-BX/ACID F5, Atago, Japan). Three independent replicates were carried out.