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2 protocols using graphitepowder

1

Graphite-based Composite Synthesis

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Graphite
powder was provided
from Alpha Chemika. Zirconium oxychloride, 1,4-benzene dicarboxylic
acid, copper sulfate, and methylene blue were purchased from MP Biomedicals,
LLC. Sodium alginate was used as received (M/G ratio of the alginate
= 1.5, Mw = 6.4 × 104 g/mol, viscosity:
200 ± 20 mPa·s 1% wt (25 °C), Shanghai McLean Biochemical
Technology Co., Ltd., China). Hydrogen peroxide, chloroacetic acid, N,N-dimethyl formamide, and hydrochloric
acid were bought from Loba Chemie. Potassium permanganate, methanol,
and ethanol were supplied by Aladdin Reagent Co., Ltd. Sodium hydroxide,
calcium chloride, sodium nitrate, and sulfuric acid were obtained
from Sinopharm Chemical Reagents.
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2

Graphene Oxide Synthesis and Characterization

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Reagents include graphite powder (Alpha Chemika, India), sulphuric acid (Loba Chemie Pvt. Ltd., Mumbai, 98%), potassium nitrate (Loba Chemie Pvt. Ltd., Mumbai, 99%), potassium permanganate (Loba Chemie Pvt. Ltd., Mumbai, 99%), hydrogen peroxide (Loba Chemie Pvt. Ltd., Mumbai, India), HCl (Molychem, India), Orange (II) (Alfa Aesa, UK), Eosin Y (Dae-Jung Chemicals & Metals Co. Ltd., Korea), sodium borohydride (Sigma Aldrich, USA) and H2O2 (Loba Chemie, India). The stock solution was made in double distilled water. Luria bertani agar (Himedia, India) used for the antibacterial assay.
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