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Hsc003 methylcellulose medium

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HSC003 methylcellulose medium is a cell culture medium that contains methylcellulose as a gelling agent. Methylcellulose is a water-soluble polymer that can be used to create a semi-solid matrix for cell culture applications.

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Lab products found in correlation

Inverted infinity and phase contrast microscope, by Thermo Fisher Scientific (2 mentions) Facsaria 2 system, by BD (2 mentions)

Close spelling variants of this product

Methylcellulose medium HSC003 Methylcellulose

2 protocols using hsc003 methylcellulose medium

1

Assessing Stem Cell Differentiation Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess differentiation potential of phenotypic pre-malignant stem
cells (preMDS/AML-SC) and malignant stem cells (MDS/AML-SCs), cells were
FACS-sorted from additional patients with the same strategy (Supplementary Fig. 1a), and plated
in HSC003 methylcellulose medium according to the manufacturer’s
recommendation (R&D Systems, Minneapolis, MN). Colonies of different
hematopoietic lineages were scored two weeks after plating using an Inverted
Infinity and Phase Contrast Microscope (Fisher Scientific, Hampton, NH). In
addition, to examine the expression of lineage makers, methylcellulose medium
was dissolved in PBS to dissociate the colonies into single cell suspension.
Cells were stained with antibodies against CD14, CD15 and CD235a on ice for 30
minutes, then analyzed on a BD FACSAria II system.
Chen J., Kao Y.R., Sun D., Todorova T.I., Reynolds D., Narayanagari S.R., Montagna C., Will B., Verma A, & Steidl U. (2018). Myelodysplastic Syndrome Progression to Acute Myeloid Leukemia at the Stem Cell Level. Nature medicine, 25(1), 103-110.
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2

Assessing Stem Cell Differentiation Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess differentiation potential of phenotypic pre-malignant stem
cells (preMDS/AML-SC) and malignant stem cells (MDS/AML-SCs), cells were
FACS-sorted from additional patients with the same strategy (Supplementary Fig. 1a), and plated
in HSC003 methylcellulose medium according to the manufacturer’s
recommendation (R&D Systems, Minneapolis, MN). Colonies of different
hematopoietic lineages were scored two weeks after plating using an Inverted
Infinity and Phase Contrast Microscope (Fisher Scientific, Hampton, NH). In
addition, to examine the expression of lineage makers, methylcellulose medium
was dissolved in PBS to dissociate the colonies into single cell suspension.
Cells were stained with antibodies against CD14, CD15 and CD235a on ice for 30
minutes, then analyzed on a BD FACSAria II system.
Chen J., Kao Y.R., Sun D., Todorova T.I., Reynolds D., Narayanagari S.R., Montagna C., Will B., Verma A, & Steidl U. (2018). Myelodysplastic Syndrome Progression to Acute Myeloid Leukemia at the Stem Cell Level. Nature medicine, 25(1), 103-110.
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+ Expand

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