Labsolution lc gc 5

All chromatographic analyses were performed using the Shimadzu Nexera UHPLC system (Kioto, Japan). This chromatograph is equipped with a binary solvent delivery system (LC-30AD), an autosampler with a 20 µL volume loop (SIL-20AC), a column thermostat (CTO20AC), and a diode-array UC-detector (SPD-M20A). LabSolution LC/GC 5.65 software (Shimadzu, Kioto, Japan) was used to collect and process data and control the apparatus. Acetonitrile (ACN) (HPLC Grade) and methanol (MeOH) (HPLC Grade) were purchased from Sigma–Aldrich (Steinheim, Germany). Packaging solvents, isopropanol, and methanol were purchased from J.T. Baker, Deventer, the Netherlands. Water was prepared with a Milli-Q Water Purification System (Millipore Corporation, Bedford, MA, USA). Standards of nucleosides—adenosine, guanosine, uridine—were obtained from Applichem (Darmstadt, Germany). Non-polar compounds—naphthalene, benzene, phenanthrene, caffeine, theophylline, and theobromine—were obtained from Sigma–Aldrich (St. Louis, MO, USA). For the synthesis of stationary phases (3-glycidoxypropyl) trimethoxysilane, decanol, octadecanol, cholesterol, benzyl alcohol, and phosphorus chloride were used, which were purchased from Alfa Aesar (Karlsruhe, Germany). Haskel (Burbank, CA, USA) laboratory equipment and packing pump were used to pack the stationary phases into 125 × 4.6 mm long empty columns.

The chromatographic assessments were conducted utilizing the Shimadzu Nexera UHPLC system (Tokyo, Japan). This system features a binary solvent delivery pump (LC-30AD), an autosampler equipped with a 20 μL volume loop (SIL-20AC), a column temperature controller (CTO20AC), and a diode-array UC-detector (SPD-M20A). Data acquisition and instrument control were managed using LabSolution LC/GC 5.65 software developed by Shimadzu in Tokyo, Japan.