Hank s balanced salts solution hbss

Cytochalasin B (CB), cytochrome c, dextran, triton-X-100, DMSO, fMLF, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), xanthine oxidase, and α-tocopherol were obtained from MilliporeSigma (St. Louis, MO, USA). Hydroethidine (HE) and Hank’s balanced salts solution (HBSS) were acquired from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Biological Industries (Beth Haemek, Israel) was the source of trypan blue. N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide was obtained from Calbiochem Research Biochemicals (La Jolla, CA, USA). We purchased 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST-1) from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). Ficoll-paque plus was procured from GE Healthcare Systems (Little Chalfont, Buckinghamshire, UK). The antibodies against protein kinase B (Akt), phospho-Akt (Ser473), phospho-p38, p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK), phospho-JNK, extracellular signal-regulated kinase (ERK), and phospho-ERK were acquired from Cell Signaling Technology (Beverly, MA, USA). The fluo-3-acetomethoxyester was acquired from Molecular Probes biotechnology company (Eugene, OR, USA). Anti-CD11b antibodies were bought from Abcam (Abcam, Cambridge, MA, USA).