The PBK and phospho-PBK (pT9) antibodies were purchased from Cell Signaling Technology (4942 and 4941). Rabbit polyclonal phospho-specific antibodies against PBK T24, S32, and S59 were generated and purified by AbMart. The peptides used for immunizing rabbits were SVLCS-pT-PTINI (T24), INIPA-pS-PFMQK (S32) and RGLSH-pS-PWAVK (S59). The corresponding non-phosphorylated peptides were also synthesized and used for antibody purification and blocking assays. Anti-Flag antibody was from Sigma. Anti-PLK1 was from BioLegend. Anti-β-actin, anti-Mps1/TTK, anti-cyclin E1, and anti-cyclin B antibodies were from Santa Cruz Biotechnology. Anti-aurora-A, anti-CDK2, anti-glutathione S-transferase (GST), anti-BUB1, and anti-BubR1 antibodies were from Bethyl Laboratories. Anti-phospho-S10 H3, anti-YAP, anti-phospho-S127 YAP, anti-phospho-S397 YAP, anti-vimentin, anti-E-cadherin, anti-N-cadherin, anti-CDC25C, anti-CDK4, anti-CDK5, anti-CDK6, anti-Cyclin A2, anti-Cyclin E2, anti-MAD2, anti-phospho-S795 Rb, anti-Wee1, and anti-phospho-S642 Wee1 antibodies were from Cell Signaling Technology. Anti-β-tubulin (Sigma) antibodies were used for immunofluorescence staining.
Anti plk1
Manufactured by BioLegend
Anti-PLK1 is a monoclonal antibody that targets the Polo-like kinase 1 (PLK1) protein. PLK1 is a serine/threonine-protein kinase that plays a crucial role in cell division and is involved in various cellular processes, including mitotic entry, centrosome maturation, and chromosome segregation. The Anti-PLK1 antibody can be used as a research tool to study the function and regulation of PLK1 in cellular and biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho s10 h3, by Cell Signaling Technology (1 mentions)
Anti phospho yap s127, by Cell Signaling Technology (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
Anti cyclin b, by Santa Cruz Biotechnology (1 mentions)
Anti cdk4, by Cell Signaling Technology (1 mentions)
Anti n cadherin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti wee1, by Cell Signaling Technology (1 mentions)
Anti β tubulin, by Merck Group (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti cyclin e2, by Cell Signaling Technology (1 mentions)
Anti cyclin a2, by Cell Signaling Technology (1 mentions)
Anti cdc25c, by Cell Signaling Technology (1 mentions)
Anti cyclin e1, by Santa Cruz Biotechnology (1 mentions)
Anti bubr1, by Fortis Life Sciences (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Anti cdk5, by Cell Signaling Technology (1 mentions)
Anti yap, by Cell Signaling Technology (1 mentions)
Anti cdk6, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti plk1
1
Antibody Characterization for PBK Phosphorylation
2
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary antibodies used were: anti-GAPDH (sc-47724, Santa Cruz), anti-AR (sc-7305, Santa Cruz), anti-DNMT3A (sc-365769, Santa Cruz), anti-EZH2 (612667, BD Transduction Laboratory), anti-DNMT(5032S, Cell Signaling Technologies), anti-EED (85322S, Cell Signaling Technologies), anti-SUZ12 (3737S, Cell Signaling Technologies), anti-Aurora A (14475T, Cell Signaling Technologies), antiH3K27me3 (9733S, Cell Signaling Technologies), and anti-PLK1 (B290751, BioLegend).
Tumor tissues (25–30 mg) or cellular pellets were lysates with RIPA buffer supplemented with cocktail phosphatase inhibitors (4906845001, Roche) and proteases inhibitors (5892953001, Roche). Protein concentration was determined by BCA reagent (A52255, Thermo Fisher Scientific); 30–50 μg of whole protein lysate was separated on 8–12% SDS–polyacrylamide gels and transferred onto PVDF membrane (88518, Thermo Fisher Scientific). The membranes were blocked with 5% milk in Tris-buffered saline with Tween-20 (TBST) for 30 min at RT, incubated overnight at 4 °C with primary antibodies, and incubated for 1 h at RT with secondary antibodies (anti-rabbit IgG HRP W401B and anti-mouse IgG HRP, W402B, Promega). The protein bands were visualized using the western bright quantum reagent (K-12042-D20, Advansta) and quantified using the Fusion Solo IV LBR system.
Tumor tissues (25–30 mg) or cellular pellets were lysates with RIPA buffer supplemented with cocktail phosphatase inhibitors (4906845001, Roche) and proteases inhibitors (5892953001, Roche). Protein concentration was determined by BCA reagent (A52255, Thermo Fisher Scientific); 30–50 μg of whole protein lysate was separated on 8–12% SDS–polyacrylamide gels and transferred onto PVDF membrane (88518, Thermo Fisher Scientific). The membranes were blocked with 5% milk in Tris-buffered saline with Tween-20 (TBST) for 30 min at RT, incubated overnight at 4 °C with primary antibodies, and incubated for 1 h at RT with secondary antibodies (anti-rabbit IgG HRP W401B and anti-mouse IgG HRP, W402B, Promega). The protein bands were visualized using the western bright quantum reagent (K-12042-D20, Advansta) and quantified using the Fusion Solo IV LBR system.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!