Anti tgf β1 monoclonal antibody mab 1d11

L-glutamine, penicillin/streptomycin, donkey anti-mouse IgG-Alexa Fluor 488, Chicken anti-rabbit IgG-Alexa Fluor 594, Laemmli sample buffer and Trizol Reagent were purchased from Invitrogen (Carlsbad, CA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 and bovine serum albumin (BSA) were from Sigma (St Louis, MO), and fetal bovine serum (FBS) from Thermo scientific. Rabbit anti α-SMA, anti-CD16 and anti-CD14 bound micromagnetic beads were purchased from Miltenyi Biotec (Auburn, CA), mouse anti-human E-cadherin antibody from BD Biosciences (Mississauga, ON, Canada), and anti-TGF-β₁ monoclonal antibody (mAb) (1D11) from R&D Systems (Minneapolis, MN). Sepasol-RNA I super G (Nacalai tesque), anti-mouse antibodies against CD11b (integrin α_M), CD49d (integrin α₄), CD29 (integrin β₁), CD18 (integrin β₁), CD54 (ICAM-1), and CD106 (VCAM-1) were from BioLegend.

L-glutamine, penicillin/streptomycin, Trizol Reagent, donkey anti-mouse IgG Alexa Fluor 488, and chicken anti-rabbit IgG-Alexa Fluor 594 were purchased from Invitrogen (Carlsbad, CA), Ham's F12K from Wako (Osaka, Japan) and fetal bovine serum (FBS) from Thermo Scientific. Anti-TGF-β1 monoclonal antibody (mAb) (1D11) from R&D Systems (Minneapolis, MN). Radioimmunoprecipitation (RIPA) lysis buffer was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase-conjugated goat anti-rabbit IgG, horseradish peroxidase conjugated goat anti-mouse IgG and Coomassie brilliant blue were from BIO-RAD (Hercules, CA, USA). Anti-CD16 and anti-CD14 bound micromagnetic beads, rabbit anti α-SMA were from Miltenyi Biotec (Auburn, CA), Sepasol-RNA I super G, gelatin, and casein from Nacalai Tesque (Kyoto, Japan) and doxycycline was from Sigma-Aldrich (St Louis, MO).