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2 protocols using anti pd l1 m1h1

1

Antibody Characterization for Immunoanalyses

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The following antibodies were used for Western Blot analyses and
immunoprecipitations: anti-HSP90: H114 (SantaCruz), anti-CMTM6: HPA026980
(Atlas) or anti-CMTM6 monoclonal antibody directed against a peptide in the
C-terminal domain of CMTM6 generated by Absea, anti-CMTM4: HPA014704 (Atlas),
anti-PD-L1 for Western blot analysis: 405.9A11 (Cell Signaling), anti-PD-L1 for
immunoprecipitation: E1L3N (Cell Signaling), normal rabbit IgG: #2729 (Cell
Signaling), anti-FLAG tag: M2 (Sigma), anti-V5 tag: R960-25 (Thermofisher),
anti-STUB1 sc 133066(Santa Cruz), anti-Ubiquitin antibody #3933 (Cell
signaling), Goat anti-mouse IgG (H+L)- HRP conjugate (BIO-RAD), and Goat
anti-rabbit IgG (H+L)- HRP conjugate (BIO-RAD). The following antibodies were
used for flow cytometry: anti-PD-L1: M1H1 (eBioscience), anti-PD-L2: 24F.10C12
(Biolegend), anti-MHC-I: BB7.2 (BD bioscience), anti-murine TCR: H57-597 (BD
bioscience), anti-CD8: RPA-T8 (BD bioscience), anti-CD3: SK7 (eBioscience),
anti-PD-1: eBioJ105 (eBioscience), anti-IL-2: 554567 (BD bioscience). The
following antibodies were used for immunohistochemistry: anti-PD-L1: 22C3
(Dako); anti-CMTM6 monoclonal antibody directed against a peptide in the
C-terminal domain of CMTM6, was generated by Absea.
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2

Antibody Characterization for Immunoanalyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for Western Blot analyses and
immunoprecipitations: anti-HSP90: H114 (SantaCruz), anti-CMTM6: HPA026980
(Atlas) or anti-CMTM6 monoclonal antibody directed against a peptide in the
C-terminal domain of CMTM6 generated by Absea, anti-CMTM4: HPA014704 (Atlas),
anti-PD-L1 for Western blot analysis: 405.9A11 (Cell Signaling), anti-PD-L1 for
immunoprecipitation: E1L3N (Cell Signaling), normal rabbit IgG: #2729 (Cell
Signaling), anti-FLAG tag: M2 (Sigma), anti-V5 tag: R960-25 (Thermofisher),
anti-STUB1 sc 133066(Santa Cruz), anti-Ubiquitin antibody #3933 (Cell
signaling), Goat anti-mouse IgG (H+L)- HRP conjugate (BIO-RAD), and Goat
anti-rabbit IgG (H+L)- HRP conjugate (BIO-RAD). The following antibodies were
used for flow cytometry: anti-PD-L1: M1H1 (eBioscience), anti-PD-L2: 24F.10C12
(Biolegend), anti-MHC-I: BB7.2 (BD bioscience), anti-murine TCR: H57-597 (BD
bioscience), anti-CD8: RPA-T8 (BD bioscience), anti-CD3: SK7 (eBioscience),
anti-PD-1: eBioJ105 (eBioscience), anti-IL-2: 554567 (BD bioscience). The
following antibodies were used for immunohistochemistry: anti-PD-L1: 22C3
(Dako); anti-CMTM6 monoclonal antibody directed against a peptide in the
C-terminal domain of CMTM6, was generated by Absea.
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