Chemical interaction between GA and PLGA through O/W emulsion was analyzed by infrared (IR) spectra. The samples, containing GA, PLGA, and GA/PLGA NPs, were freeze-dried in preparation. Fourier-transform IR spectra were recorded on a Nicolet Nexus 470-ESP (Thermo Fisher Scientific, Waltham, MA, USA). Baseline values were corrected, and the samples were scanned against a blank KBr-pellet background at wave numbers in the range of 400–4,000 cm−1.
The diameter and polydispersity of the NPs in (0.1 M, pH 7.4) were measured by dynamic light scattering (DLS) using a BI-900AT/ζ-potential analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA). To evaluate the stability of NPs, the RBCm-GA/PLGA NPs were kept at 25°C and the diameter determined by DLS every day for a week.
The structure of the RBCm-GA/PLGA NPs was examined using transmission electron microscopy (JEM-100S; JEOL, Tokyo, Japan). A drop of the NP suspension was deposited onto a cupper grid and air-dried at room temperature. The sample was stained with 1% uranyl acetate before observation. Drug release was studied by dialyzing samples against PBS at different pH values (7.4 and 5.4) using a dialysis bag with a molecular weight cutoff of 10 kDa at 37°C for a week. The GA content was detected using HPLC.
The diameter and polydispersity of the NPs in (0.1 M, pH 7.4) were measured by dynamic light scattering (DLS) using a BI-900AT/ζ-potential analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA). To evaluate the stability of NPs, the RBCm-GA/PLGA NPs were kept at 25°C and the diameter determined by DLS every day for a week.
The structure of the RBCm-GA/PLGA NPs was examined using transmission electron microscopy (JEM-100S; JEOL, Tokyo, Japan). A drop of the NP suspension was deposited onto a cupper grid and air-dried at room temperature. The sample was stained with 1% uranyl acetate before observation. Drug release was studied by dialyzing samples against PBS at different pH values (7.4 and 5.4) using a dialysis bag with a molecular weight cutoff of 10 kDa at 37°C for a week. The GA content was detected using HPLC.