Ebc 1

GC cell lines MKN-45 and MKN-28, and immortalized human gastric epithelial cell line GES-1, were obtained from 3D Biopharm Biotech Co. Ltd. (Shanghai, China). NCI-N87, HGC-27, AGS, and SGC-7901 cell lines were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). SNU-216 and GTL-16 cell lines were gifts from the Medical College of Xiamen University (Xiamen, China) and AstraZeneca China R&D Center (Shanghai, China). EBC-1 cell line was obtained from COBIOER BIOSCIENCES Co. Ltd (Nanjing, China). Cell lines were tested and authenticated by short tandem repeat DNA profiling analysis before execution of the experiments. Cells were cultured in Minimum Essential Medium (HGC-27), F12K medium (AGS), Dulbecco's Modified Eagle's Medium (GTL-16), Eagle's Minimum Essential Medium (EBC-1) or Roswell Park Memorial Institute 1640 medium containing 10% fetal bovine serum (Gibco, Carlsbad, CA, USA) and 1% penicillin-streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C in a humidified atmosphere with 5% carbon dioxide.

Human lung adenocarcinoma cell lines A549 (Cat no. SCSP-503), NCI-H1975 (Cat no. TCHu193) and NCI-H460 (H460, Cat no. TCHu205), BEAS-2B human bronchial epithelial cells (Cat no. SCSP-5067), and 293T cells (Cat no. SCSP-502) were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). 95-D human lung squamous carcinoma cell line was obtained from the American Type Culture Collection (ATCC). EBC-1 human lung squamous carcinoma cell line was obtained from the Japanese Research Resources Bank (Tokyo, Japan). BEAS-2B cells are normal lung epithelial cells, and the BEAS-2B cell line is regarded as a “control” for LC cells. In follow-up experiments, the present study mainly focused on using the A549 and H1975 cell lines, as the clinicopathological types of LC represented by these two cell lines are more common. A549, H1975, H460, 95D, and EBC-1 cells were cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Inc., Cat no. 11875093) containing 10% fetal bovine serum (Gibco, Cat no. 16140071) and 1% penicillin-streptomycin (Gibco, Cat no. 15140148). BEAS-2B normal lung epithelial and 293T cells were cultured using DMEM (Gibco, Cat no. 11965092) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. All cells were maintained in an incubator at 37°C, in an atmosphere with 5% CO₂ and saturated humidity.