Sodium dichloroisocyanurate, trichloroisocyanuric acid, 4-chloro-3,5-dimethylphenol, McLean Biochemical Technology Co., Ltd., Shanghai, China; terbium chloride(TbCl3), Nycodenz, tea polyphenols (catechin content accounted for 41.31%, epicatechin content accounted for 19.72%, quercetin content accounted for 3.71%, and gallic acid content accounted for 4.75%), Macklin Biochemical Technology Co., Ltd., Shanghai, China; nisin, nitrite, potassium sorbate, D-isoascorbic acid, sodium diacetate, ԑ-polylysine hydrochloride, Sigma-Aldrich Trading Co., Ltd., Shanghai, China; tryptose Sulfite cycloserine Agar Base (TSC), thioglycollate medium (FTG), Hi-tech Industrial Park Haibo Biotechnology Co., Ltd., Shandong, China; whey protein fermentation powder, yinong biology science and technology Co., Ltd., Shanghai, China; Other reagents such as glutaraldehyde, silver nitrate standard solution and hydrogen peroxide solution are analytically pure in China.
D isoascorbic acid
Manufactured by Merck Group
Sourced in China, Germany, United States
D-isoascorbic acid is a chemical compound that functions as an antioxidant. It is an isomer of the more commonly known vitamin C (ascorbic acid). D-isoascorbic acid is used in various laboratory applications, including as a reducing agent and an additive in cell culture media.
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Lab products found in correlation
Nisin, by Merck Group (1 mentions)
Sodium diacetate, by Merck Group (1 mentions)
Potassium sorbate, by Merck Group (1 mentions)
N n methylenebisacrylamide, by Merck Group (1 mentions)
Cetyltrimethylammonium bromide, by Thermo Fisher Scientific (1 mentions)
Oleic acid, by Thermo Fisher Scientific (1 mentions)
Acrylic acid, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
N isopropylacrylamide, by Merck Group (1 mentions)
Chloroauric acid, by Merck Group (1 mentions)
Didecyldimethylammonium bromide, by Merck Group (1 mentions)
Cytochrome c cyt c, by Merck Group (1 mentions)
Uv vis detector, by Shimadzu (1 mentions)
Arsenic trioxide, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
4 protocols using d isoascorbic acid
1
Antimicrobial Agents and Food Preservation
2
Synthesis of Stimuli-Responsive Protein-Polymer Conjugates
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O-[2-(3-Mercaptopropionylamino)ethyl]-O′-methylpolyethylene glycol (PEG-SH) of molecular weight 5000 Da, N-isopropylacrylamide (NIPAm), N-N′-Methylene-bisacrylamide (BIS), Acrylic acid (AAc), potassium persulfate (KPS), Didecyldimethylammonium bromide (DDAB, 98%), Chloroauric acid (HAuCl4·3H2O, 99.999%), d -(−)-Isoascorbic acid (AsA, 98%), Sodium borohydride (NaBH4, ≥96%), and Cytochrome C (Cyt C) samples were purchased from Sigma-Aldrich® (Schnelldorf, Germany). Cetyltrimethylammonium bromide (CTAB, 99%+) and Sodium citrate dihydrate (Na-citrate, ACS grade) were obtained from Acros Organics® (Geel, Belgium) and Merck® (Darmstadt, Germany), respectively. Oleic acid (OA, 90%) was purchased from Alfa Aesar (Karlsruhe, Germany). All the chemicals were used as received without any further purification or modification. All solutions were prepared using distilled de-ionized water (MQ-water), having a resistivity ~18.2 MΩ/cm at 25 OC, taken from Simplicity® Millipore (Darmstadt, Germany ) water purification system.
3
Quantitative HPLC Analysis of Ascorbic Acid
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The HPLC system used for the AA detection was as follows: HPLC liquid chromatograph pump, UV/VIS detector, column oven, auto sampler, degassing unit, and communications bus module (all Shimadzu) with HPLC column XBridge Amide 3.5 μm, 4.6 × 150 mm (Waters) and Guard column XBridge Amide 3.5 μm, 4.6 × 20 mm (Waters, Milford, MA, USA). The UV detection was at 255 nm wavelength. The mobile phase contained 85% acetonitrile and 15% water with 10 mM NH4Ac pH 7. The flow rate was 1 mL/min, the pressure 63 bar, the temperature of column oven 25 °C, the temperature of the auto sampler was 19 °C, and the injection volume was 20 μL. The concentration of vitamin C in the sample was specified using a calibration curve prepared beforehand using known concentrations of AA (Sigma Aldrich, Saint Louis, MO, USA) and D-(-) Isoascorbic acid (Sigma Aldrich, Saint Louis, MO, USA).
4
Preparing Vitamin C and Arsenic Trioxide Solutions
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The Ascorbic Acid (VC, catalogue #A7506), d-(-) isoAscorbic Acid (D-VC, catalogue #856061), and Arsenic Trioxide (ATO, catalogue #A1010) used in our study was obtained from Sigma. To prepare 340 mM stock solution of VC, 30 g of D-VC was dissolved in sterile 400 ml PBS solution following the addition of 39 g of sodium bicarbonate (NaHCO3, catalogue #S5761). The solution was constantly stirred for another 5 minutes. Sodium bicarbonate was used as a buffer to bring the acidic (2.3) pH of VC up to physiological levels (pH 7.35) for injection into laboratory animals. The final D-VC solution was adjusted to 500 ml by adding PBS. The D-VC solution was filtered, aliquoted, and kept in a -20°C refrigerator to ensure that the drug was as pure and effective as possible. The stock solution of 330 mM ATO was prepared by mixing and dissolving 13 g of ATO in 1 N sodium hydroxide (NaOH). Then, the total volume of ATO was adjusted to 200 ml. The final solution was filtered and stored in a -20°C refrigerator.
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