Mouse monoclonal anti gapdh clone 71

FITC-conjugated goat anti-rat antibody and, unlabeled and PE-conjugated mouse anti-human CRIg monoclonal antibody (clone 6H8, 1:200 for Western blotting), were from Santa Cruz Biotechnology (Dallas, TX). Fluorochrome-conjugated antibodies to CD45 (APC-H7; clone 2D1), CD11b (PE; clone D12), and mouse IgG1-κ isotype control (PE; clone MOPC-21), were from BD Biosciences (Franklin Lakes, NJ). The rabbit anti-ARPC1B antibody (HPA004832, 1:1000) and mouse monoclonal anti-GAPDH (clone 71.1, 1:20,000) were from Sigma-Aldrich. The polyclonal HRP-conjugated rabbit anti-mouse (P0260), anti-goat (P0449), and goat anti-rabbit (P0448) immunoglobulin antibodies (1:2000) were purchased from Dako (Glostrup, Denmark). The polyclonal anti-myeloperoxidase (MPO) antibody was purchased from Abcam (Cambridge, UK). The Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody was purchased from Invitrogen (Waltham, MA).

The mouse monoclonal antibody (clone 3C9, for flow cytometry, 0.2 µg; for western blotting, 1:3000) that recognizes the IgV domain of human CRIg was kindly provided by Dr. Menno van Lookeren Campagne (Genentech, San Francisco, CA). The rabbit recombinant monoclonal anti-CD11b antibody (ab133357, clone EPR1344, 1:1000), and mouse IgG1 isotype control antibody (ab37355) were purchased from Abcam. The mouse monoclonal anti-CD11c antibody (clone N-19, 1:1000) and goat PE-conjugated anti-mouse IgG antibody were purchased from Santa Cruz Biotechnology. The mouse monoclonal anti-GAPDH (clone 71.1, 1:20,000) was obtained from Sigma-Aldrich. The polyclonal HRP-conjugated rabbit anti-mouse (P0260), anti-goat (P0449) and goat anti-rabbit (P0448) immunoglobulin antibodies (1:2000) were obtained from Dako.