Ab75762

Manufactured by Abcam

Ab75762 is a laboratory equipment product manufactured by Abcam. The core function of this product is to assist in scientific research and experimentation, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab15552, by Merck Group (2 mentions) Ab189034, by Abcam (1 mentions) Ab54481, by Abcam (1 mentions) Ab15251, by Abcam (1 mentions) Ab2893, by Abcam (1 mentions) Vector m o m immunodetection kit, by Vector Laboratories (1 mentions) Af1819, by R&D Systems (1 mentions) Sc 9068, by Santa Cruz Biotechnology (1 mentions) Ab32572, by Abcam (1 mentions)

2 protocols using ab75762

Histological Analysis of Mouse Kidney

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Paraffin‐embedded mouse kidney sections (4 µm thickness) were prepared by a routine procedure (Luo et al., 2018). Masson trichrome staining (BA‐4079A; BASO) and Sirius red staining (DC0040; Leagene Biotechnology) were performed according to the manufacturer's protocol. Immunohistochemical staining was performed as described previously (Zhou et al., 2017). After incubation with specific antibodies, kidney sections were stained using Vector M.O.M. Immunodetection Kit according to the protocol specified by the manufacturer (Vector Laboratories). For a negative control, the primary antibody was omitted, and no staining occurred. Antibodies used were as follows: Klotho (AF1819; R&D Systems), Wnt1 (ab15251; Abcam), AT1 (AB15552; Merck Millipore), ACE (ab75762; Abcam), AGT (SAB2100072; Sigma‐Aldrich), p16^INK4A (ab189034; Abcam), γ‐H2AX (ab2893; Abcam), PGC‐1α (ab54481; Abcam), and DKK1 (BM4554; Boster).

Miao J., Liu J., Niu J., Zhang Y., Shen W., Luo C., Liu Y., Li C., Li H., Yang P., Liu Y., Hou F.F, & Zhou L. (2019). Wnt/β‐catenin/RAS signaling mediates age‐related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell, 18(5), e13004.

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Western Blot Analysis of Kidney Proteins

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Protein expression in kidney homogenates was analyzed by Western blot analysis as described previously [17 (link)]. The primary antibodies used were as follow: rabbit polyclonal anti-fibronectin (sc-9068; Santa Cruz Biotechnology), rabbit polyclonal anti-collagen I (234167, EMD Millipore), rabbit monoclonal anti-β-catenin antibody (ab32572; Abcam, Cambridge, MA), and rabbit monoclonal anti-β-actin (4970, Cell Signaling Technology), rabbit polyclonal anti-AT1 receptor (ab15552; Millipore, Billerica, MA), rabbit monoclonal anti-ACE (ab75762; abcam, Cambridge, MA).

Xiao L., Xu B., Zhou L., Tan R.J., Zhou D., Fu H., Li A., Hou F.F, & Liu Y. (2019). Wnt/β-catenin regulates blood pressure and kidney injury in rats. Biochimica et biophysica acta. Molecular basis of disease, 1865(6), 1313-1322.

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