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Anti phospho her2 tyr 1248

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The Anti-phospho HER2 (Tyr 1248) is a laboratory reagent used to detect the phosphorylated form of the HER2 protein at tyrosine residue 1248. It is a highly specific antibody that can be used in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study the activation and regulation of the HER2 signaling pathway.

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Lab products found in correlation

Anti p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Anti phospho p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Anti her2, by Cell Signaling Technology (1 mentions) Anti plcγ1, by Cell Signaling Technology (1 mentions) Anti smyd3, by Cell Signaling Technology (1 mentions) Anti actb, by Cell Signaling Technology (1 mentions) Anti histone h3, by Abcam (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Anti egfr, by Cell Signaling Technology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti β actin 13e5, by Cell Signaling Technology (1 mentions) Nupage 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Anti phospho tyrosine, by Cell Signaling Technology (1 mentions) Ubiquitin p4d1, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti p53, by Cell Signaling Technology (1 mentions) Anti phospho pak1, by Cell Signaling Technology (1 mentions) Anti mcl 1, by Cell Signaling Technology (1 mentions) Anti bcl xl, by Cell Signaling Technology (1 mentions) Anti phospho akt thr308, by Cell Signaling Technology (1 mentions)

3 protocols using anti phospho her2 tyr 1248

1

Immunoblotting of Receptor Tyrosine Kinase Signaling

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The following primary antibodies were used: anti‐FLAG (rabbit, F7425; Sigma‐Aldrich; dilution used in WB: 1:1000), anti‐HA (rabbit, Y‐11; Santa Cruz Biotechnology; Santa Cruz, CA; dilution used in ICC: 1:1000), anti‐SMYD3 (rabbit, D2Q4V; Cell Signaling Technology; Danvers, MA; dilution used in WB: 1:1000), anti‐HER2 (rabbit, 29D8; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐phospho HER2 (Tyr 1248) (rabbit, #2247; Cell Signaling Technology; dilution used in WB: 1:500), anti‐EGFR (rabbit, D38B1; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐ACTB (rabbit, #4967; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐histone H3 (rabbit, ab1791; Abcam; Cambridge, UK; diluted used in: 1:1000), anti‐AKT (rabbit, C67E7; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐phospho AKT (Ser 473) (mouse, 587F11; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐PLCγ1 (rabbit, D9H10; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐phospho PLCγ1 (Tyr 783) (rabbit, #2821; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐p44/42 MAPK (Erk1/2) (rabbit, #9102; Cell Signaling Technology; dilution used in WB: 1:1000), anti‐phospho p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (rabbit, D13.14.4E; Cell Signaling Technology; dilution used in WB: 1:1000).
Yoshioka Y., Suzuki T., Matsuo Y., Tsurita G., Watanabe T., Dohmae N., Nakamura Y, & Hamamoto R. (2017). Protein lysine methyltransferase SMYD3 is involved in tumorigenesis through regulation of HER2 homodimerization. Cancer Medicine, 6(7), 1665-1672.
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2

Immunoblot Analysis of HER2 Signaling

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Total protein lysates (20 μg) were extracted using RIPA buffer and separated on SDS-PAGE gels (NuPAGE™ 4–12% Bis-Tris Protein Gels, Invitrogen) according to standard methods.
Membranes were probed using the following antibodies: anti-total HER2 Rabbit mAb (29D8, Cell Signalling #2165), anti-total HER2 Mouse mAb (CB11, Thermo Scientific #MA1–35720), anti-phospho-HER2 (Tyr1248, Cell Signalling #2247), anti-Cleaved PARP Asp214 human specific (Cell Signalling Technology #9541), anti-phospho-tyrosine (Cell Signaling Technology #8954), anti-β-Actin 13E5 (Cell Signalling Technology #4970) and Ubiquitin (P4D1, Cell Signaling Technology #3936S).
Li B.T., Michelini F., Misale S., Cocco E., Baldino L., Cai Y., Shifman S., Tu H.Y., Myers M.L., Xu C., Mattar M., Khodos I., Little M., Qeriqi B., Weitsman G., Wilhem C.J., Lalani A.S., Diala I., Freedman R.A., Lin N.U., Solit D.B., Berger M.F., Barber P.R., Ng T., Offin M., Isbell J.M., Jones D.R., Yu H.A., Thyparambil S., Liao W.L., Bhalkikar A., Cecchi F., Hyman D.M., Lewis J.S., Buonocore D.J., Ho A.L., Makker V., Reis-Filho J.S., Razavi P., Arcila M.E., Kris M.G., Poirier J.T., Shen R., Tsurutani J., Ulaner G.A., de Stanchina E., Rosen N., Rudin C.M, & Scaltriti M. (2020). HER2-mediated internalization of cytotoxic agents in ERBB2 amplified or mutant lung cancers. Cancer discovery, 10(5), 674-687.
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3

Regulation of MUC13, HER2, and Apoptosis Pathways

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Cells were transfected with miR-145 mimics, NC in presence or absence of miR-145 inhibitor (Assay id MH11480; Applied Biosystems) and total protein was extracted from PanCa cells, followed by Western blotting as previously described [30 (link), 33 (link)]. Proteins were analyzed by immunoblotting with anti-MUC13 MAb (clone PPZ020), anti-HER2 (catalog number A0485; DAKO), anti-phospho-HER2 (tyr1248) (catalog number 2247; Cell Signaling), anti-phospho-PAK1 (catalog number 2605; Cell Signaling), anti-p44/42 MAPK (ERK1/2) (catalog number 9102; Cell Signaling), PAK1 (catalog number 2602; Cell Signaling), anti-phospho-p44/42 MAPK (ERK1/2) (catalog number 9101; Cell Signaling), anti-AKT (catalog number 9272; Cell Signaling), anti-phospho-AKT (Thr308) (catalog number 2965; Cell Signaling), anti-p53 (catalog number 2527; Cell Signaling), anti caspase-3 (catalog number 9662; Cell Signaling), anti-Bcl-xL (catalog number 2764; Cell Signaling), anti Mcl-1 (catalog number 5453; Cell Signaling), GAPDH (catalog number 5174; Cell Signaling) and anti-β–Actin (Sigma).
Khan S., Ebeling M.C., Zaman M.S., Sikander M., Yallapu M.M., Chauhan N., Yacoubian A.M., Behrman S.W., Zafar N., Kumar D., Thompson P.A., Jaggi M, & Chauhan S.C. (2014). MicroRNA-145 targets MUC13 and suppresses growth and invasion of pancreatic cancer. Oncotarget, 5(17), 7599-7609.
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