Bi cat elisa

Manufactured by ALPCO

The Bi-CAT ELISA is a laboratory equipment designed for enzyme-linked immunosorbent assay (ELISA) testing. It provides a platform for the detection and quantification of specific analytes in a sample.

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Lab products found in correlation

Milliplex map kit, by Merck Group (1 mentions) Bio plex pro human diabetes 12 plex panel, by Bio-Rad (1 mentions)

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ELISAs (7 citations)

2 protocols using bi cat elisa

Multiplex Biomarker Quantification

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Adiponectin, adipsin, ghrelin, glucose-dependent insulinotropic polypeptide (GIP), GLP-1, glucagon, leptin, resistin, and visfatin were measured with the Bio-Plex Pro-Human-Diabetes multiplex immunoassay panels (BioRad). Epinephrine and Norepinephrine were quantified using a direct competitive enzyme immunoassay (Bi-CAT ELISA, Alpco Diagnostics, Salem, NH). Lactate concentrations were determined colorimetrically by coupled enzymatic reactions using commercial assay kits (Sigma). Triiodothyronine (T3) was measured using the rat thyroid hormone panel Milliplex MAP kit (Millipore; there is no difference between rat and human T3).

Wandrag L., Siervo M., Riley H.L., Khosravi M., Fernandez B.O., Leckstrom C.A., Martin D.S., Mitchell K., Levett D.Z., Montgomery H.E., Mythen M.G., Stroud M.A., Grocott M.P, & Feelisch M. (2017). Does hypoxia play a role in the development of sarcopenia in humans? Mechanistic insights from the Caudwell Xtreme Everest Expedition. Redox Biology, 13, 60-68.

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Diabetes Biomarker Quantification

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Glucagon was measured with the Bio-Plex-Pro-Human-Diabetes 12-plex panel (BioRad). Adrenalin and Noradrenalin were quantified using a direct competitive enzyme immunoassay (Bi-CAT ELISA, Alpco Diagnostics, Salem, NH).

Siervo M., Riley H.L., Fernandez B.O., Leckstrom C.A., Martin D.S., Mitchell K., Levett D.Z., Montgomery H.E., Mythen M.G., Grocott M.P, & Feelisch M. (2014). Effects of Prolonged Exposure to Hypobaric Hypoxia on Oxidative Stress, Inflammation and Gluco-Insular Regulation: The Not-So-Sweet Price for Good Regulation. PLoS ONE, 9(4), e94915.

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