Avance drx 600 mhz spectrometer

Optical rotations were acquired on a digital DIP-370 polarimeter (JASCO). The UV spectra were measured on a Hitachi 300 spectrometer. NMR data were acquired on a Bruker Avance DRX 600 MHz spectrometer using CD₃OD as the solvent. Positive ion HRESIMS spectra were collected on a Thermo LTQ Orbitrap XL mass spectrometer. A SiO₂ RP HPLC column (Merck, 70–230 mesh ASTM) and Sephadex LH 20 (0.25–0.1 mm, Pharmacia) were used for chromatography. An HPLC column (AR II Cosmosil, Waters, 250 × 10 mm, 5 μm) was used for purification of the compounds.

Optical rotation was measured on the automatic high-speed laboratory polarimeter P3000 (A.KRUSS Optronic Gmbh, Hamburg, Germany). UV spectra were measured on a Hitachi 300 Spectrophotometer (Hitachi High-Technologies Corporation, Kyoto, Japan). High-resolution ESIMS data were recorded with an ultra-high resolution (UHR) TOF spectrometer (Impact, Bruker, Bremen, Germany). NMR spectra were obtained in CDCl₃ on a Bruker Avance DRX 600-MHz spectrometer (Bruker, Bremen, Germany) at 600 MHz for ¹H-NMR and 150 MHz for ¹³C-NMR. NMR chemical shifts were expressed in parts per million (ppm) referenced to residual CDCl₃ solvent signals (δ_H 7.26 for ¹H and δ_C 77.0 for ¹³C). Precoated SiO₂ 60 F₂₅₄ plates (Merck, Darmstadt, Germany) were used for TLC. For column chromatography, SiO₂ (70–230 mesh, Merck, Darmstadt, Germany) was used. HPLC purifications were performed on an HPLC column (5-µm ZORBAX Eclipse XDB-C18, 250 × 4.6 mm, Agilent, Santa Clara, CA, USA).

Optical rotations values were measured on a digital DIP-370 polarimeter (JASCO, Oklahoma City, OK, USA), while the UV spectra are obtained on a Hitachi 300 spectrometer (Hitachi High-Technologies Corporation, Kyoto, Japan). The ECD spectra were obtained on a JASCO J-810 spectropolarimeter (JASCO, Oklahoma City, OK, USA) with a 0.5 cm cell in MeOH. The NMR spectra were acquired on Bruker Avance DRX 600 MHz spectrometer (Bruker, Rheinstetten, Germany). Positive ion HRESIMS spectra were obtained on a Thermo LTQ Orbitrap XL mass spectrometer (Thermo Finnigan, Bremen, Germany). Normal SiO₂ (Merck, Darmstadt, Germany) and Sephadex LH 20 (Pharmacia, Uppsala, Sweden) were used for chromatography.