Tes buffer

Manufactured by Merck Group

Sourced in France

TES buffer is a common laboratory reagent used to maintain a stable pH in various biological applications. It is a zwitterionic buffer that aids in maintaining the desired pH environment for experiments or sample preparation. The core function of TES buffer is to provide a stable and consistent pH environment for experiments and processes requiring a controlled pH level.

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Lab products found in correlation

Clostridial collagenase, by Merck Group (1 mentions) Isopropanol, by Merck Group (1 mentions) Tri reagent, by Euromedex (1 mentions) Ethanol, by Carlo Erba (1 mentions) Chloroform, by Carlo Erba (1 mentions)

Spelling variants of this product

TE buffer (43 citations) Tris-EDTA buffer (TE) (4 citations) Chloramine-T/citrate buffer solution (3 citations) Chloramine T/Oxidation Buffer Mixture (3 citations) TE buffer solution (2 citations) TE buffer (Ph 8.0; (2 citations) T-PER buffer (2 citations)

3 protocols using tes buffer

Collagenase Digestion of Bone Particles

Cited 1 time

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BP collagenase digestion stability was performed as previously described [37 (link)]. Briefly, Clostridial collagenase (Sigma C9722) was dissolved in 50 mM TES Buffer (Sigma T1375) to a concentration of 600 U/mL with the addition of 0.36 mM calcium chloride. Collagenase digestion was performed for 24 hours at 37°C on lyophilized BP samples following a 28-day incubation in PBS with or without inhibitors. Following the treatment, digestion was measured as a percentage of dry weight loss.

Osuga J.I., Ishibashi S., Oka T., Yagyu H., Tozawa R., Fujimoto A., Shionoiri F., Yahagi N., Kraemer F.B., Tsutsumi O, & Yamada N. (2000). Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity. Proceedings of the National Academy of Sciences of the United States of America, 97(2), 787-792.

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CLD Exposure Protocol for Hydra

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Pure CLD was provided as a powder from Azur Isotopes (Marseille, France), with a purity greater than 97 %, and was diluted in pure water at a concentration of 1 mg/L. All the solutions were stored in the dark at 4°C. The stock solutions of CLD for expositions were freshly prepared in TES buffer (Sigma-Aldrich, Saint Quentin-Fallavier, France), the hydra breeding media. Isopropanol was purchased from Sigma (L'Isle d'Abeau, France). Chloroform (99%), and ethanol (75%) were from Carlo Erba® Reagents (Val-de-Reuil, France).
Moloney Murine Leukemia Virus Reverse Transcriptase was from In Vitrogen/Thermo-Fischer (Cergy-Pontoise, France). PCR Water Nuclease Free, 5x HOT Pol Evagreen ® qPCR Mix Plus, and Tri-Reagent™ were from Euromedex (Souffelweyersheim, France).

Colpaert R., Villard P.H., de Jong L., Mambert M., Benbrahim K., Abraldes J., Cerini C., Pique V., Robin M, & Moreau X. (2020). Multi-scale impact of chronic exposure to environmental concentrations of chlordecone in freshwater cnidarian, Hydra circumcincta. Environmental science and pollution research international, 27(33).

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Hydra Clone Culturing for Toxicity Study

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The hydra clone (strain IMBE1) was not collected in FWI. As far as we know, this clone has been used in several laboratories in North America and Europe since at least the last four decades (first mention in the literature by Johnson in 1980) and has not been exposed to CLD previously. Therefore, it could not have developed tolerance to CLD. The hydra clone (strain IMBE1) was reared in TES buffer (0.1 mM; pH 7) (Sigma-Aldrich, Saint Quentin-Fallavier, France) at 20 ± 0.1 °C and under a 12/12 h light-dark cycle according to the procedure of De Jong et al. (2016) (link) which was adapted from Trottier et al. (1997) (link). Polyps were fed ad libitum every three to four days with nauplii of Artemia sp. hatched within 24 h. All specimens used in this experiment were derived from asexual reproduction and belong to the same clone.

Moreau X., Claeys-Bruno M., Andraud J.P., Macarie H., Martínez D.E., Robin M., Sergent M, & De Jong L. (2022). Hydra bioassay for the evaluation of chlordecone toxicity at environmental concentrations, alone or in complex mixtures with dechlorinated byproducts: experimental observations and modeling by experimental design. Environmental science and pollution research international, 29(60).

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