Lcms 8040 liquid

Plant samples were washed with ultrapure water, air-dried, and chopped. The sample was weighed to 500 mg, and 1.5 mL of extraction solvent (methanol/water/formic acid, 80:18:2; v/v/v) was added. After 60 min of ultrasonic extraction at 50 °C, the samples were centrifuged at 14,000 rpm for 8 min [26 (link)]. The supernatant was filtered through a 0.22 μm filter and centrifuged at 19,000 rpm for 5 min. The samples were dried in a metal bath at 50 °C with a nitrogen blower and reconstituted by adding an equal volume of methanol. A total of 200 μL of the reconstituted samples was transferred to the sample vials with 250 μL lined tubes, and the Shimadzu LCMS-8040 liquid chromatography–mass spectrometer was used for LC-MS/MS detection [32 (link),33 (link)]. Soil samples were processed as above.

Polyphenols of RCSF1 were quantitatively analyzed using a LCMS-8040™ liquid chromatography mass spectrometer (Shimadzu, Kyoto, Japan) with an electrospray ionization probe and a Nexera UHPLC system equipped with a phenyl-hexyl column (2.1 × 100 mm, 3.5 μm; Agilent, Santa Clara, CA, USA). Detailed multiple reaction monitoring conditions of target analytes are indicated in Table S1. Standard polyphenols purchased from Sigma-Aldrich were identified as previously reported [11 (link)].