Cells were washed with ice-cold PBS and lysed with radioimmunoprecipitation (RIPA) lysis buffer (Thermo Fisher Scientific, 89900) at the end of the experimental time point. Before proteins were run on SDS-PAGE gels and transferred to PVDF membranes, concentrations were ascertained using a BCA protein assessment kit (Thermo Fisher Scientific, Waltham, MA, USA). Antibodies including anti-L13a (ab96074) and anti-L11 (ab79352) used to probe the blots were acquired from Abcam (Cambridge, MA, USA). Anti-phospho L13a antibody was generated as previously described (42 (link)). Anti-NS1, anti-NS2, and anti-M antibodies were produced by AtaGenix (Wuhan, China). To confirm the equal protein loading for all the conditions tested, blots were immunoblotted using β-Actin antibody (ab8226) purchased from Abcam(Cambridge, MA, USA).
β actin antibody ab8226
Manufactured by Abcam
Sourced in United States
β-Actin antibody (ab8226) is a primary antibody that specifically recognizes the β-actin protein, which is a widely expressed and highly conserved cytoskeletal protein. This antibody can be used in various applications to detect and quantify β-actin levels.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Sc79 s7863, by Selleck Chemicals (1 mentions)
P akt ser 473 antibody 9271, by Cell Signaling Technology (1 mentions)
Mhcc 97h cells, by National Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using β actin antibody ab8226
1
Western Blot Analysis of Key Proteins
2
Huh7 and MHCC-97H Cell Culture and Drug Treatment
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Huh7 and MHCC-97H cells were purchased from Cell Bank of Chinese Academy of Sciences and cultured in DMEM (06-1055-57-1ACS, Biological Industries) with 10% fetal bovine serum (04-002-1A, Biological Industries), penicillin (100U/mL), streptomycin (10μg/mL) at 37◦C with 5% CO2 in a humidified incubator. Pirarubicin (S1393), necrostatin-1 (S8037) and SC79 (S7863) were purchased from Selleck. RIPK1 antibody (ab72139), P21 antibody (ab109520), β-actin antibody (ab8226) and GAPDH antibody (ab8245), Rb antibody (ab181616) and pRbS780 antibody (ab173289) were purchased from abcam. AKT antibody (4691) and p-AKTSer473 antibody (9271) were purchased from Cell Signaling Technology. Goat anti-Mouse (A00160) and anti-Rabbit HRP (A00166) were purchased from GenScript.
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