Nucleic acid sequencing was carried out on a PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems). Sequencing primers were as previously reported.(10 (link)) Comparison to reported immunoglobulin germline sequences from Genbank/EMBL/DDBJ was carried out using NCBI blast against Ig sequences (Ig blast ) (http://www.ncbi.nlm.nih.gov/igblast/ ) and IMGT/V-QUEST (http://www.imgt.org/IMGT_vquest/share/textes/ ) searches.
Prism 310 genetic analyzer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PRISM 310 Genetic Analyzer is a capillary electrophoresis instrument designed for DNA sequencing and fragment analysis. It utilizes a single capillary to perform high-resolution separations of DNA fragments. The PRISM 310 Genetic Analyzer is capable of performing a variety of genetic analysis applications, including DNA sequencing, microsatellite analysis, and single nucleotide polymorphism (SNP) detection.
Automatically generated - may contain errors
2 protocols using prism 310 genetic analyzer
1
Immunoglobulin Sequence Analysis Protocol
2
Screening for IDH1/2 and H3F3A Mutations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To screen for IDH1/2, H3F3A mutations, we performed high-resolution melting (HRM) analysis using DNA extracted from frozen tissue as described previously [7, 8] . Subsequently, we used direct sequencing to determine the base sequences of the mutations. The PCR products obtained from the HRM analysis were diluted 50-fold and purified using ExoSAP-IT (Affymetrix/USB, Santa Clara, CA, USA), after which cycle sequencing was performed using BigDye® Terminator v3.1 Cycle Sequencing kits (Applied Biosystems, Tokyo, Japan). Following purification with a BigDye XTerminator® Purification Kit (Applied Biosystems), electrophoresis and analysis were conducted using a PRISM ® 310 Genetic Analyzer (Applied Biosystems). The BRAF V600E mutations were analyzed in 359 samples and TERT promoter mutations were analyzed by sequencing for 325 samples as described previously [8] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!