Curcumol, N-acetyl cysteine (NAC), N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-FMK), necrotatin-1 (Nec-1), and SP600125 were brought from Sigma-Aldrich (St Louis, MO, USA). Dulbecco's modified essential medium (DMEM), fetal bovine serum (FBS), Opti MEM medium, phosphate buffered saline (PBS), and trypsin-EDTA were purchased from GIBCO BRL (Grand Island, NY, USA). Oxidation sensitive 2′,7′-dichlorodihydrofluorescin diacetate (DCFH-DA) and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) were obtained by Beyotime Biotechnology (Shanghai, China). MitoSox Red was brought from Thermo Fisher Scientific (Waltham, MA, USA). Anti-rabbit IgG, anti-mouse IgG and antibodies against β-actin, and RIPK1 (17519-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, IL, USA). Main antibodies against α-SMA, α1(I) procollagen, EGFR, fibronectin, MLKL, PDGF-βR, p-MLKL, p-RIPK3, RIPK3, and TGF-βRII were obtained from Abcam Technology (Abcam, Cambridge, UK). Primary antibodies against caspase3, cleaved-caspase3, caspase8, cleaved-caspase8, caspase9, cleaved-caspase9, ERK, JNK, p38, p-ERK, p-JNK, p-p38, p-RIPK1 and TIMP2 were procured by Cell Signaling Technology (Danvers, MA, USA). Lentivirus vectors encoding negative control shRNA (NC shRNA) and RIPK3 shRNA were designed by Hanbio (Shanghai, China).
Necrotatin 1 nec 1
Manufactured by Merck Group
Sourced in Sao Tome and Principe
Necrotatin-1 (Nec-1) is a laboratory-grade chemical compound. It functions as a specific inhibitor of the Receptor-Interacting Serine/Threonine-Protein Kinase 1 (RIPK1), an essential mediator of necroptosis, a form of programmed cell death. Nec-1 is commonly used in research applications to study cellular mechanisms and pathways related to necroptosis.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 8, by Cell Signaling Technology (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Curcumol, by Merck Group (1 mentions)
Fibronectin, by Abcam (1 mentions)
Anti mouse igg, by Proteintech (1 mentions)
Tgf βrii, by Abcam (1 mentions)
5 5 6 6 tetrachloro 1 1 3 3 tetraethyl benzimidazolyl carbocyanineiodide jc 1, by Beyotime (1 mentions)
Timp 2, by Cell Signaling Technology (1 mentions)
Ripk3, by Abcam (1 mentions)
P ripk3, by Abcam (1 mentions)
Caspase 8, by Cell Signaling Technology (1 mentions)
α sma, by Abcam (1 mentions)
Anti rabbit igg, by Proteintech (1 mentions)
Primary antibodies against caspase 3, by Cell Signaling Technology (1 mentions)
P ripk1, by Cell Signaling Technology (1 mentions)
Pdgfβr, by Abcam (1 mentions)
N benzyloxycarbonyl val ala asp fluoromethylketone zvad fmk, by Merck Group (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Sp600125, by Merck Group (1 mentions)
Mitosox red, by Thermo Fisher Scientific (1 mentions)
2 protocols using necrotatin 1 nec 1
1
Investigating Curcumol-Mediated Cellular Responses
2
Neuroprotective Effects of Methylprednisolone
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Methylprednisolone was obtained from the Nanjing Hospital affiliated to Nanjing University of Chinese Medicine (Nanjing, Jiangsu China) and was dissolved in PBS to a concentration of 30 mg/ml and was stored at 4°C. Necrotatin-1 (Nec-1) and 4-hydroxy-TEMPO (Tempol) were bought from Sigma-Aldrich (St Louis, MO, United States). Dulbecco’s modified essential medium (DMEM), fetal bovine serum (FBS), Opti-MEM medium, phosphate-buffered saline (PBS), and trypsin-EDTA were purchased from GIBCO (Grand Island, NY, United States). The antibodies against β-actin (20536-1-AP), RIPK1 (17519-1-AP), and RIPK3 (17563-1-AP) were purchased from Proteintech Group, Inc (Rosemont, IL, United States). Caspase-3 (#9662), cleaved-caspase-3 (#9664), caspase-8 (#9746), cleaved-caspase-8 (#9748), TNF-α (#11948), IL-18 (#54943), IL-1β (#12242), and IL-6 (#12153) were procured from Cell Signaling Technology (Danvers, MA, United States). Mixed lineage kinase domain-like protein (MLKL; ab243142) were purchased from Abcam Technology (Abcam, Cambridge, United Kingdom).
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