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Rat anti mouse anti cd45r b220 apc

Manufactured by BD
Sourced in Switzerland

The Rat anti-Mouse anti-CD45R/B220-APC is a fluorescently-labeled antibody that binds to the CD45R/B220 antigen expressed on mouse B cells. It is used for the identification and analysis of B cells in flow cytometry applications.

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2 protocols using rat anti mouse anti cd45r b220 apc

1

Flow Cytometric Immunophenotyping of Mouse Immune Cells

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The following antibodies were used for flow cytometric analysis: rat anti-CD4-PE-Cy7 (BD PharmingenTM, Allschwil, Switzerland, #552775; 1:100); rat anti-CD8-PE-Cy5 (BD PharmingenTM, Allschwil, Switzerland, #553034; 1:100); anti-CD19-PE-Cy7 (Biolegend, San Diego, CA, USA, #115520; 1:100); hamster anti-mouse anti-CD3-FITC (BD PharmingenTM, Allschwil, Switzerland, #561827; 1:100); rat anti-mouse anti-CD43-FITC (BD PharmingenTM, Allschwil, Switzerland, #561856; 1:100); rat anti-mouse anti-CD45R/B220-APC (BD PharmingenTM, Allschwil, Switzerland; #553092; 1:100); rat anti-mouse anti-IgM-PE-Cy7 (BD PharmingenTM, Allschwil, Switzerland, #552867; 1:100); rat anti-mouse IgG1-APC (BD PharmingenTM, Allschwil, Switzerland; #550874; 1:100). A LIVE/DEAD™ fixable violet dead cell stain kit (ThermoFisher Scientific, Waltham, MA, USA; #L34955; 1:1000) was used to identify dead cells.
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2

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometric analysis: rat anti-CD4-PE-Cy7 (BD Pharmingen TM , Allschwil, Switzerland, #552775; 1:100); rat anti-CD8-PE-Cy5 (BD Pharmingen TM , Allschwil, Switzerland, #553034; 1:100); anti-CD19-PE-Cy7 (Biolegend, San Diego, CA, USA, #115520; 1:100); hamster anti-mouse anti-CD3-FITC (BD Pharmingen TM , Allschwil, Switzerland, #561827; 1:100); rat anti-mouse anti-CD43-FITC (BD Pharmingen TM , Allschwil, Switzerland, #561856; 1:100); rat antimouse anti-CD45R/B220-APC (BD Pharmingen TM , Allschwil, Switzerland; #553092; 1:100); rat antimouse anti-IgM-PE-Cy7 (BD Pharmingen TM , Allschwil, Switzerland, #552867; 1:100); rat anti-mouse IgG1-APC (BD Pharmingen TM , Allschwil, Switzerland; #550874; 1:100). A LIVE/DEAD™ fixable violet dead cell stain kit (ThermoFisher Scientific, Waltham, MA, USA; #L34955; 1:1000) was used to identify dead cells.
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