Male specific pathogen-free Kun-Ming mice (35–40 g) were provided by the Experimental Animal Center of Shenyang Pharmaceutical University (Shenyang, China). All the studies were performed in compliance with the Guideline for Animal Experimentation and the protocol was subject to approval by the Animal Ethics Committee of Shenyang Pharmaceutical University. All animals were housed under conventional conditions with appropriate temperature (22 ± 0.5°C), humidity (50–60%) control and a 12/12 h light/dark cycle with free access to food and water.
Spinosin (purity>99.0%) was provided by the National Institutes for Food and Drug Control of Pharmaceutical and Biological Products (Shenyang, China) and was suspended in physiological saline solution at concentrations of 0.2 and 2 µg/µl. Recombinant human Aβ1–42 (Sigma-Aldrich, St Louis, MO, USA) was dissolved and diluted in physiological saline to a stock concentration of 1.0 mg/mL, which was then sealed and incubated for 120 h at 37°C to allow the peptide to aggregate (Mao et al., 2015 (link)). Commercial kits for detection of MDA, B-cell lymphoma-2 (Bcl-2), BNDF, interleukin-6 (IL-6) and Aβ1–42 were purchased from Nanjing Jiancheng Bio engineering Institute (Nanjing, China).
Spinosin (purity>99.0%) was provided by the National Institutes for Food and Drug Control of Pharmaceutical and Biological Products (Shenyang, China) and was suspended in physiological saline solution at concentrations of 0.2 and 2 µg/µl. Recombinant human Aβ1–42 (Sigma-Aldrich, St Louis, MO, USA) was dissolved and diluted in physiological saline to a stock concentration of 1.0 mg/mL, which was then sealed and incubated for 120 h at 37°C to allow the peptide to aggregate (Mao et al., 2015 (link)). Commercial kits for detection of MDA, B-cell lymphoma-2 (Bcl-2), BNDF, interleukin-6 (IL-6) and Aβ1–42 were purchased from Nanjing Jiancheng Bio engineering Institute (Nanjing, China).