Animals were given water with BrdU (0.8 mg/ml) (Sigma, Saint Louis, MO) for 3 days after injection and then animals were sacrificed and soleus were removed 14 d after immobilization. Muscles were fixed and incubated with 2 N HCl for 20 min. Samples were washed and sections were incubated with anti-BrdU (1:200; Sigma, Saint Louis, MO) and anti-Pax7 (1:200, Abcam) at 4°C overnight, followed by Alexa 594-conjugated goat anti-mouse IgG (1:500, Abcam) and Alexa 488-conjugated goat anti-rabbit IgG (1:500, Abcam). Finally, the sections were incubated with DAPI (Life Technologies, Japan) for 1 min, washed in PBS, and mounted in mounting solution (Thermo Fisher Scientific). Positive cells were counted under 20 high-power fields (HPFs) of 25 sections and the relative number of Pax7/BrdU-positive cells in HPF was noted.
Alexa 594 conjugated goat anti mouse igg
Manufactured by Abcam
Sourced in United Kingdom
Alexa Fluor 594-conjugated goat anti-mouse IgG is a secondary antibody used for detection and visualization of mouse primary antibodies in various immunoassays and imaging applications. The antibody is conjugated to the fluorescent dye Alexa Fluor 594, which has excitation and emission spectra suitable for detection using standard rhodamine filter sets.
Automatically generated - may contain errors
Lab products found in correlation
Alexa 488 conjugated goat anti rabbit igg, by Abcam (3 mentions)
Lsm 880, by Zeiss (2 mentions)
Mounting solution, by Thermo Fisher Scientific (1 mentions)
Anti brdu, by Merck Group (1 mentions)
Bromodeoxyuridine (brdu), by Merck Group (1 mentions)
Anti pax7, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Anti ki67, by Abcam (1 mentions)
Triton x 100, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Confocal microscope, by Olympus (1 mentions)
Anti cd31 antibody, by Abcam (1 mentions)
Paraformaldehyde (pfa), by Beyotime (1 mentions)
Immunohistochemical blocking solution, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Yesen (1 mentions)
3 protocols using alexa 594 conjugated goat anti mouse igg
1
Muscle Stem Cell Proliferation Assay
2
Immunophenotyping of Glioblastoma Cells
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To identify the GBM2 isolated from glioma patients, the sample cells were incubated with anti-CD133 (1:200, Beyotime,Shanghai,China) and anti-glial fibrillary acidic protein (GFAP) (1:100, Beyotime,Shanghai,China) antibodies, or anti-CD31 antibody (rabbit immunoglobulin [IgG], 1:1000; Abcam) and anti-Ki67 (1:500, Abcam) antibodies. The cultured cells were fixed with 4% paraformaldehyde (Beyotime, Shanghai, China) for 10 min and washed three times with PBS. The cells were permeabilized with 0.1% Triton-X 100 (Beyotime, Shanghai, China) for 20 min, and blocked with 2% BSA at room temperature (RT) for 60 min. The cells were then incubated with the primary antibodies for 60 min at RT. After rinsing with PBS three times, cells were incubated with the following secondary antibodies for 60 min: Alexa 488-conjugated goat antirabbit IgG and Alexa 594-conjugated goat antimouse IgG (1:2000, Abcam,Cambridge,UK) at RT. Cell nuclei were counter-stained with DAPI (Beyotime, Shanghai, China). Cells were examined under a Confocal Microscope (Olympus,Tokyo, Japan).
3
Immunohistochemical Analysis of Wound Bed
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Samples from the wound bed on day 3 were first dewaxed and rehydrated and then repaired antigen via boiling in a 100 ℃ citrate buffer water bath for 25 min. The tissue sections were blocked with immunohistochemical blocking solution (Beyotime, China) for 90 min. The primary antibodies used in this experiment were incubated at 4 ℃ overnight, as follows: CD86 (Signalway Antibody, USA) and CD163 (GeneTex, USA) and then incubated with the following secondary antibodies for 90 min at room temperature: Alexa 488-conjugated goat anti-rabbit IgG (Abcam, UK) and Alexa 594-conjugated goat anti-mouse IgG (Abcam, UK). The nuclei were stained with DAPI (YESEN, China). Images were acquired using a laser-scanning confocal microscope (Carl Zeiss LSM880, Germany).
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