Ge illustra rna kit

Manufactured by GE Healthcare

The GE Illustra RNA kit is a laboratory product designed for the extraction and purification of RNA from various sample types. It provides a standardized and efficient method for isolating RNA for downstream analysis applications.

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Lab products found in correlation

C100 thermal cycler, by Bio-Rad (2 mentions) Cfx96 real time detection system, by Bio-Rad (2 mentions) Sybr green supermix, by Bio-Rad (2 mentions) Iscript, by Bio-Rad (2 mentions)

2 protocols using ge illustra rna kit

Gene Expression Analysis in Brassica Seedlings

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Expression analysis was performed on four whole seedlings per genotype collected at the end of the day on day 10. A 120 mg (FW) aliquot of each sample was immediately frozen in liquid nitrogen and stored at –80 °C until processing. Frozen tissue was ground in liquid nitrogen and total RNA was extracted using the GE Illustra RNA kit (GE Life Sciences), and 2 µg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad). Samples were analyzed using SYBR Green Supermix (Bio-Rad) reactions run in a C100 Thermal Cycler (Bio-Rad) fitted with a CFX96 Real-Time Detection System (Bio-Rad). Relative expression levels were calculated using the formula (E_target)^−CPtarget/(E_ref)^−CPref (Pfaffl, 2001 (link)) and normalized to the B. rapa PP2A (Brara.F00691) reference gene. qPCR primer sequences are as follows: PP2A (forward 5'-TCGGTGGTAACGCCCCCGAT-3'; reverse 5'-CGACTCTCGTGGTTCCCTCGC-3'); for PHYB (Brara.E02473) (forward 5'-GCGCTCAGAGGGGACGAGGA-3'; reverse 5'-GCGGTGTTCCACTCCAGGCA-3'); for BrSIG6 (Brara.D02249) (forward 5'-ACGCTTGCCGGAGAACGTGTA-3'; reverse 5'-ACACCAACGTGGCCTGCGAG-3'); BrGLK1 (Brara.I04696) (forward 5'-AAGCCACCCGCTGTTGACGG-3'; reverse 5'-GCCTCAGG CGCTTGTCCGTT-3'); BrGH3-5 (Brara.K00097) (forward 5'-GACCC TTACACCGACTACAC-3'; reverse 5'-CATAAGCCACAAAGCAT CTGAG-3'); BrIAA19 (Brara.C03574) (5'-TCTCGATAAGCTCTT CAGTTTCC-3'; reverse 5'-CAGTCTCCATCTTTGTCTTCGT-3').

Arsovski A.A., Zemke J.E., Haagen B.D., Kim S.H, & Nemhauser J.L. (2018). Phytochrome B regulates resource allocation in Brassica rapa. Journal of Experimental Botany, 69(11), 2837-2846.

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Quantitative Expression Analysis of Arabidopsis Genes

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Expression analysis was performed using 4 biological replicate samples collected identically as described for RNA sequencing. Each sample was immediately frozen in liquid nitrogen and stored at -80 °C until processing. Frozen tissue was ground in liquid nitrogen and total RNA was extracted using the GE Illustra RNA kit (GE Life Sciences), and 2 µg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad). Samples were analyzed using SYBR Green Supermix (Bio-Rad) reactions run in a C100 Thermal Cycler (Bio-Rad) fitted with a CFX96 Real-Time Detection System (Bio-Rad). Relative expression levels were calculated using the formula (Pfaffl, 2001) and normalized to the B.rapa PP2A (Brara.F00691) reference gene. qPCR primer sequences are as follows: BrPP2A
(forward 5'-TCGGTGGTAACGCCCCCGAT-3'; reverse 5'-CGACTCTCGTGGTTCCCTCGC-3'); BrMGT6 (Brara.E02300) (forward 5'-CAGCATCCGCCACCGCAAGA-3'; reverse 5'-GCCTTCGCAACAACCGCAGC-3');
BrRLK4 (Brara.I00004) (forward 5'-TCCGCCGTCGCGATCTCTCT-3'; reverse 5'-CCCGCTCCAAACGCTTGTCCA-3'); BrPIFI (forward 5'-GCCACCACTCTTGCACCCCC-3'; reverse 5'-CCGCGGTTGGAGGAAGACCG-3').

Arsovski A.A., Zemke J.E., Hamm M.O., Houston L., Romanowski A., Halliday K., Nési N., & Nemhauser J.L. (2020). BrphyB is critical for rapid recovery to darkness in mature Brassica rapa leaves.

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