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Mouse monoclonal anti α tubulin fitc antibody f2168

Manufactured by Merck Group

The Mouse monoclonal anti-α-tubulin-FITC antibody (F2168) is a fluorescently labeled primary antibody that specifically binds to the α-tubulin protein, a component of the cytoskeleton in eukaryotic cells. This antibody is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate), which allows for the visualization and detection of α-tubulin in various cell biology applications.

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2 protocols using mouse monoclonal anti α tubulin fitc antibody f2168

1

Antibodies for Protein Interaction Assays

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The goat polyclonal anti-TRAIP (sc-99695), anti-Mad2 (sc-6329) and mouse monoclonal anti-securin (sc-398471) antibodies were purchased from Santa Cruz Biotechnology, rabbit monoclonal anti-β-actin (4970) was brought from Cell Signal Technology, mouse monoclonal anti-BubR1 antibody (ab54894) was from Abcam, mouse monoclonal anti-α-tubulin-FITC antibody (F2168) was obtained from Sigma.
CREST, human anti-centromere antibody (HCT-0100) was brought from Immunovision. Anti-γH2AX antibody (05-636) was from Millipore.
FITC or TRITC conjugated goat anti-mouse, rabbit anti-goat antibodes and goat anti-human antibodies were bought from Zhongshan Golden Bridge Biotechnology (ZF-0314, ZF-0317, ZF-0313, ZF-0308). All other reagents were bought from Sigma Aldrich except when noted otherwise.
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2

Antibodies for Cell Signaling Analysis

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Mouse monoclonal anti‐Myc antibody (ab32), rabbit monoclonal anti‐ARP2 antibody (ab128934), rabbit anti‐N‐WASP monoclonal antibody (ab126626), rabbit monoclonal anti‐GM130 antibody (ab52649), rabbit monoclonal anti‐ROCK1 antibody (ab45171) (for western blot) and rabbit polyclonal anti‐RAB14 antibody (ab28639) were purchased from Abcam. A rabbit polyclonal anti‐ROCK1 antibody (bs‐1166R) (for immnuofluorescence staining) was purchased from Bioss. Mouse monoclonal anti‐α‐tubulin‐FITC antibody (F2168), the protease from Streptomyces griseus (P8811), phalloidin‐Atto 590 (93042) and Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) (B2261) were obtained from Sigma. Rabbit monoclonal anti‐α‐tubulin antibody (#2125) and rabbit monoclonal phosphor‐cofilin (Ser3) antibody (#3313) were purchased from Cell Signaling Technology. Golgi‐Tracker Red (C1043) was obtained from Beyotime. Alexa Fluor 488 goat anti‐Rabbit antibody (ZF‐0511), and Alexa Fluor 594 goat anti‐Rabbit antibody (ZF‐0516) were purchased from Zhongshan Golden Bridge Biotechnology.
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