The OVCAR3 and OVCAR8 OC cell lines, both widely regarded to originate from HGSC, were obtained from the American Type Culture Collection (ATCC) and cultured according to the manufacturer’s instructions. OVCAR3 cells were cultured in DMEM, OVCAR8 in RPMI (Biological Industries, Beit-Haemek, Israel). The medium was supplemented with 1% L-glutamine, 1% sodium pyruvate, 1% vitamin solution, 1% non-essential amino acids (Biological Industries) and 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO, USA). Cells were grown in a humidified atmosphere of 95% air and 5% CO2. In cells from which exosomes were extracted, the medium used was EXCELL® Advanced™ CHO Fed-batch Medium (Sigma-Aldrich). OVCAR3 and OVCAR8 OC spheroids were formed by constant shaking of 400,000 cells per well in 6-well plates for 48 h on a vertical shaker.
Ovcar8
Manufactured by Sartorius
Sourced in Israel
OVCAR8 is a laboratory cell line derived from human ovarian cancer. It is commonly used in research studies related to ovarian cancer.
Automatically generated - may contain errors
Lab products found in correlation
Vitamin solution, by Sartorius (2 mentions)
Fetal calf serum (fcs), by Merck Group (2 mentions)
Non essential amino acids (neaa), by Sartorius (2 mentions)
Roswell park memorial institute (rpmi), by Sartorius (2 mentions)
Ex cell advanced cho fed batch medium, by Merck Group (2 mentions)
L glutamine, by Sartorius (2 mentions)
Ovcar3, by American Type Culture Collection (2 mentions)
Ovcar8, by American Type Culture Collection (2 mentions)
Sodium pyruvate, by Sartorius (1 mentions)
Ovcar3, by Sartorius (1 mentions)
Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions)
2 protocols using ovcar8
1
Ovarian Cancer Cell Line Culture
2
Culturing OVCAR3 and OVCAR8 Ovarian Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The OVCAR3 and OVCAR8 OC cell lines were obtained from the American Type Culture Collection (ATCC) and cultured according to the manufacturer's instructions. OVCAR3 cells were cultured in DMEM, OVCAR8 in RPMI (Biological Industries, Beit-Haemek, Israel). The medium was supplemented with 1% L-glutamine, 1% sodium pyruvate, 1% vitamin solution, 1% non-essential amino acids (Biological Industries) and 10% fetal calf serum (Sigma-Aldrich, St.
Louis MO). Cells were grown in a humidified atmosphere of 95% air and 5% CO2. In cells from which exosomes were extracted, the medium used was EX-CELL ® Advanced ™ CHO Fed-batch Medium (Sigma-Aldrich).
Louis MO). Cells were grown in a humidified atmosphere of 95% air and 5% CO2. In cells from which exosomes were extracted, the medium used was EX-CELL ® Advanced ™ CHO Fed-batch Medium (Sigma-Aldrich).
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