Te 11

Human lung cancer cell lines (H1299, 95-D, SPCA-1, A549, SK-MES-1, PC-9, H2170 and Hcc-827), human esophageal cancer cell lines (TE-11 and EC-109), human normal esophageal cell lines (HET-1A and HEEpiC), human acute monocytic leukemia cell line (THP-1), and human breast cancer cell line (MCF-7) were purchased from the Cell Bank of the China Academy of Science (Shanghai, China). THP-1, EC-109 and all the human lung cancer cell lines were grown in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gemini, Woodland, CA, USA), 100 IU/ml penicillin and 100 µg/ml streptomycin. TE-11 and MCF-7 cells were grown in Dulbeccos modified Eagles medium (DMEM) culture medium (Life Technologies) supplemented with 10% FBS, 100 IU/ml penicillin and 100 µg/ml streptomycin. HET-1A and HEEpiC were grown in EpiCM-2 complete medium (ScienCell, Carlsbad, CA, USA) with 100 IU/ml penicillin and 100 µg/ml streptomycin. All of the cells were cultured at 37°C in a humidified incubator with 5% CO₂.