The normal gastric epithelial cell line GES-1 and GC cell lines (AGS, HGC27, MKN45, and SGC-7901) were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China). All cell lines were incubated with RPMI 1640 medium (Solarbio, Beijing, China) supplemented with 10% FBS (Solarbio) in a humidified incubator at 37 °C containing 5% CO2.
FAM120A, WTAP, METTL3, METTL14, YTHDC1, SLCTA11, and PD-L1 knockdown and overexpression lentiviruses and their respective control vectors were generated by GenePharma (Shanghai, China). GC cells were inoculated into 6-well dishes and then infected with lentivirus at 60% confluence. Stable transfected cells were produced by treating cells with puromycin (4 μg/ml) for 2 weeks. The shRNA sequences are listed in Supplementary Table2 .
FAM120A, WTAP, METTL3, METTL14, YTHDC1, SLCTA11, and PD-L1 knockdown and overexpression lentiviruses and their respective control vectors were generated by GenePharma (Shanghai, China). GC cells were inoculated into 6-well dishes and then infected with lentivirus at 60% confluence. Stable transfected cells were produced by treating cells with puromycin (4 μg/ml) for 2 weeks. The shRNA sequences are listed in Supplementary Table