For the migration assay, 8μm transwell plates (Corning-Costar) were used. Briefly, cells were seeded in the upper chamber and chemoattractant EGF (100ng/ml) was added to the lower chambers as previously described. 12 hours after EGF stimulation, cells that migrated to the lower chamber were fixed, stained using Hema stain and counted. For the invasion assay, pre-coated Matrigel invasion chambers (BD Falcon) were used. After 24 hours of stimulation with EGF similar to that in migration assay, invaded cells were stained and counted. Wound scratch assay was done as described previously [49 (link)].
Pre coated matrigel invasion chambers
Manufactured by BD
Pre-coated Matrigel invasion chambers are a type of laboratory equipment used to assess the invasive potential of cells. They consist of a porous membrane coated with a basement membrane extract, Matrigel, which serves as a barrier to cell migration. The chambers allow for the evaluation of a cell's ability to invade through the Matrigel layer, which is a key step in the process of metastasis.
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Lab products found in correlation
2 protocols using pre coated matrigel invasion chambers
1
Cell Migration and Invasion Assays
2
Cell Migration and Invasion Assays
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